Acetyl CoA Carboxylase 1, Rabbit anti-Human, Polyclonal, Millipore Sigma™

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma ABS106825UL

Catalog No. ABS106825UL

Description

Anti-Acetyl CoA Carboxylase 1, Cat. No. ABS1068, is a rabbit polyclonal antibody that detects Acetyl CoA Carboxylase 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Acetyl-CoA carboxylase 1 (UniProt: Q13085; also known as EC: 6.4.1.2, ACC1, ACC-alpha) is encoded by the ACACA (also known as ACAC, ACC1, ACCA) gene (Gene ID: 31) in human. ACC1 is a cytoplasmic protein that is expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues. It is also expressed at low level in pulmonary tissue, but is not detected in the liver. ACC1 can be present as a monomer, homodimer, or homotetramer. It can also form filamentous polymers. Four isoforms of ACC1 have been described that are produced by alternative splicing. It catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids and carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. ACC1 levels are regulated under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Mutations in ACACA gene can result in ACC1 deficiency and lead to error of de novo fatty acid synthesis associated with severe brain damage, persistent myopathy and poor growth.

Specifications

• Antigen: Acetyl CoA Carboxylase 1
• Classification: Polyclonal
• Dilution: Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Acetyl CoA Carboxylase 1 in human liver and human kidney tissue sections.
• Gene Alias: ACC1;ACC-alpha;EC 6.4.1.2
• Host Species: Rabbit
• Molecular Weight of Antigen: ∽220 kDa observed; 265.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
• Quantity: 25 μL
• Research Discipline: Signaling
• Test Specificity: This rabbit polyclonal antibody detects Acetyl-CoA carboxylase 1 in human cells. It targets an epitope within 245 amino acids from the C-terminal region.
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Conjugate: Unconjugated
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Symbols: ACACA;ACC1;ACCA
• Immunogen: GST-tagged recombinant fragment corresponding to 245 amino acids from the C-terminal half of human Acetyl-CoA carboxylase 1.
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human