Invitrogen™ H3K9ac Recombinant Rabbit Monoclonal Antibody (17H12L11)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ 701269

Catalog No. PI701269

Description

701269 was successfully used to detect H3K9ac in WB, IF, IHC, ChIP and flow applications. This antibody is predicted to react with mouse, rat, non-human primate and rabbit based on sequence homology. Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain. Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specifications

• Antigen: H3K9ac
• Classification: Recombinant Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide
• Gene Accession No.: P68431, Q71DI3
• Gene Symbols: H3C1, H3C10, H3C12, H3C14, H3C15, H3C2, H3C7, His3:CG31613
• Immunogen: Acetylated peptide corresponding to amino acids 6-12 of human Histone H3 [AcK9].
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Rat, Drosophila
• Product Type: Antibody
• Isotype: IgG

• Applications: Peptide Array, Western Blot, Immunocytochemistry
• Clone: 17H12L11
• Conjugate: Unconjugated
• Gene: H3C15
• Gene Alias: Acetyl H3; Acetyl Histone H3; Acetyl-H3 K9; Acetyl-H3 Lys9; Acetyl-Histone H3 K9; Acetyl-Histone H3 Lys9; B0035.7; BUR5; CELE_T10C6.12; CG31613; CG31613-PA; CG33803; CG33806; CG33809; CG33812; CG33815; CG33818; CG33821; CG33824; CG33827; CG33830; CG33833; CG33836; CG33839; CG33842; CG33845; CG33848; CG33851; CG33854; CG33857; CG33860; CG33863; CG33866; Dmel\CG31613; Dmel_CG31613; F07B7.10; F07B7.3; F08G2.2; F17E9.13; F35H10.1; F45F2.4; F54E12.5; F55G1.10; H02I12.7; H3; H3 histone; H3 histone family, member A; H3 histone family, member I; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3 histone, family 3A; H3 histone, family 3B; H3 histone, family 3B (H3.3B); H3 histone, family 3B.1; H3 histone, family 3C; H3 K9ac; H3.1-221; H3.1-291; H3.1-I; H3.2; H3.2-221; H3.2-614; H3.2-615; H3.2-616; h3.2a; H3.3A; H3.3B; H3.5; H3/A; H3/b; H3/d; H3/f; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3-143; H3-291; H3-3A; H3-3B; h3-5; H3-53; H3-614; H3a; H3b; H3-B; H3C1; H3c10; H3c11; H3C12; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3f; H3-F; H3F1K; H3F2; H3F3; H3F3A; H3F3B; h3f3b.1; H3F3C; h3f3d; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; H3g; H3h; H3i; H3K9; H3Lys9ac; h3r; HHT1; HHT2; his-12; his-16; his-19; his-21; His3; his-3; His3:CG31613; His3:CG31613-PA; His3:CG33803; His3:CG33806; His3:CG33809; His3:CG33812; His3:CG33815; His3:CG33818; His3:CG33821; His3:CG33824; His3:CG33827; His3:CG33830; His3:CG33833; His3:CG33836; His3:CG33839; His3:CG33842; His3:CG33845; His3:CG33848; His3:CG33851; His3:CG33854; His3:CG33857; His3:CG33860; His3:CG33863; His3:CG33866; his-30; his-33; his-43; his-47; his-51; his-53; his-57; his-61; his-65; his-68; his-7; Hist1; HIST1H3A; HIST1H3B; Hist1h3c; HIST1H3D; HIST1H3E; Hist1h3f; hist1h3g; hist1h3g.L; HIST1H3H; Hist1h3i; HIST1H3J; hist2h3; HIST2H3A; Hist2h3b; HIST2H3C; Hist2h3c1; Hist2h3c2; Hist2h3c2-ps; Hist2h3ca1; Hist2h3ca2; HIST2H3D; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 2, H3a; histone 2, H3c; histone 2, H3c2; histone 2, H3ca2; Histone 3; histone cluster 1, H3a; histone cluster 1, H3b; histone cluster 1, H3f; histone cluster 1, H3g protein L homeolog; histone cluster 1, H3h; histone cluster 2, H3a; histone cluster 2, H3c; histone cluster 2, H3c2; histone cluster 2, H3c2, pseudogene; histone gene complex 1; Histone H2A; Histone H3; histone H3.1; Histone H3.2; histone H3.3; histone H3.3A; histone H3.3C; Histone H3.5; histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; histone H3/l; Histone H3/m; histone H3/o; Histone H3K9; Histone H3K9ac; histone variant H3.5; hypothetical protein LOC550262; K06C4.11; K06C4.3; M32461; N2749; PP781; SIN2; T10C6.12; T23D8.6; wu:fa25h06; wu:fa96g06; wu:fb07a08; wu:fb36f01; XELAEV_18028537mg; YBR010W; YBR0201; YNL031C; zgc:110292; zgc:174300; zgc:56193; zgc:56418; zgc:64222; zgc:86731; ZK131.10; ZK131.6
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 126961, 318847, 333932, 8350, 8356, 8357, 8358, 8968
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid