Actin Muscle Monoclonal Antibody (HHF35), Invitrogen™

Catalog No.	Quantity
PIMA514084	500 μL

Catalog No. PIMA514084 Supplier Thermo Scientific Supplier No. MA514084

Description

Mouse Monoclonal Antibody

This antibody reacts with actin from tissue extracts of uterus, ileum, aorta, diaphragm, heart and smooth muscle cells. It recognizes alpha-actin from skeletal, cardiac, and smooth muscle, and reacts with gamma actin from smooth muscle. This antibody will react with tumors from smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle tumors (rhabdomyomas and rhabdomyosarcomas). Staining of formalin-fixed tissues requires boiling in 1mM EDTA, pH 8.0, followed by cooling at room temperature for 20 min.

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

Specifications

• Antigen: Actin Muscle
• Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
• Classification: Monoclonal
• Clone: HHF35
• Concentration: Conc. Not Determined
• Conjugate: Unconjugated
• Formulation: tissue culture supernatant with 0.09% sodium azide
• Gene: ACTA1
• Gene Accession No.: P62736, P62737, P62738, P63259, P63260, P63261, P63267, P63268, P63269, P68032, P68033, P68035, P68133, P68134, P68136
• Gene Alias: 0610041G09Rik; AA959943; a-actin; AAT6; ACT; Act4; ACT-4; ACTA; ACTA1; ACTA1 protein; Acta2; Acta-2; ACTA3; ACTB; Actc; ACTC1; Actc-1; ACTE; Actg; Actg1; Actg2; ACTGE; actin; actin alpha 1; actin alpha 1, skeletal muscle; actin alpha 2, smooth muscle; actin alpha cardiac; actin alpha cardiac 1; actin alpha cardiac muscle 1; actin gamma 1; actin gamma 2, smooth muscle; actin homolog; actin, alpha 1, skeletal muscle; actin, alpha 2, smooth muscle, aorta; actin, alpha cardiac muscle 1; Actin, alpha cardiac muscle 1, intermediate form; Actin, alpha skeletal muscle; Actin, alpha skeletal muscle, intermediate form; actin, alpha, cardiac 1; actin, alpha, cardiac muscle; actin, alpha, cardiac muscle 1; actin, alpha, vascular smooth muscle; actin, aortic smooth muscle; Actin, aortic smooth muscle, intermediate form; actin, beta; actin, cytoplasmic 2; Actin, cytoplasmic 2, N-terminally processed; Actin, cytoplasmic type 5; actin, cytoplasmic, type 5; actin, gamma 1; actin, gamma 1 propeptide; actin, gamma 2; actin, gamma 2, smooth muscle, enteric; actin, gamma, cytoplasmic 1; actin, gamma-enteric smooth muscle; Actin, gamma-enteric smooth muscle, intermediate form; actin-like protein; Actl; ACTL3; Acts; ACTSA; Actsg; Actsk-1; Actvs; AL023024; alpha actin; alpha actin 1; alpha actin 2; alpha-actin; alpha-actin cardiac; alpha-actin-1; Alpha-actin-2; alpha-actin-3; alphac-actin; Alpha-cardiac actin; alphaSMA; alpha-sma; alpha-smooth muscle actin; ASD5; ASMA; a-SMA; B-actin; beta actin; beta-actin; beta-actin FE-3; BRWS2; cardiac actin; cardiac alpha actin; cardiac muscle alpha actin 1; cell growth-inhibiting gene 46 protein; CFTD; CFTD1; CFTDM; CMD1R; CMH11; cytoplasmic 2; cytoplasmic actin; cytoplasmic beta-actin; cytoplasmic type 5; cytoskeletal gamma-actin; deafness, autosomal dominant 20; deafness, autosomal dominant 26; DFNA20; DFNA26; E51; epididymis luminal protein 176; gamma 1 propeptide; gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; GIG46; HEL-176; hypothetical protein LOC515610; LOW QUALITY PROTEIN: actin, alpha skeletal muscle; LVNC4; MPFD; MYMY5; NEM1; NEM2; NEM3; nemaline myopathy type 3; RP5-1068B5.2; SHPM; similar to b-actin; skeletal alpha actin; skeletal muscle alpha-actin 1; SMalphaA; SMGA; smooth muscle alpha-actin; smooth muscle gamma-actin; type 5; unnamed protein product; vascular smooth muscle alpha-actin; VSCM
• Gene Symbols: ACTA1, ACTA2, ACTC1, ACTG1, ACTG2
• Host Species: Mouse
• Immunogen: SDS extract of human myocardium
• Quantity: 500 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 11459, 11464, 11465, 11468, 11475, 25365, 287876, 29275, 29437, 58, 59, 70, 71, 72, 81633
• Target Species: Human, Mouse, Rat
• Content And Storage: 4° C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ