MilliporeSigma™ Actin, Rabbit, Alexa Fluor 647, Polyclonal,

Catalog No.	Quantity	Conjugate
ABT1485AF64	100 μL	Alexa Fluor 647

Catalog No. ABT1485AF64 Supplier MilliporeSigma™ Supplier No. ABT1485AF647

Description

Rabbit Polyclonal Antibody

Actins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin (ACTA1), smooth muscle alpha-actin (ACTA2), cytoplasmic beta-actin (ACTB), cardiac muscle alpha-actin (ACTC1), cytoplasmic gamma-actin (ACTG1), and smooth muscle gamma-actin (ACTG2, a.k.a. ACTA3). Although actins show more than 90% overall sequence homology, isotypes do show spatial, temporal, and tissue-specific expresson patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic β and γ-actins are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt. In additon to serving as the basic building blocks of cytoskeletal microfilaments, actins are involved in multiple nuclear functions and adopt specific conformations that are important for their association with nuclear actin-binding proteins.

Specifications

• Antigen: Actin
• Applications: Immunocytochemistry, Immunofluorescence
• Classification: Polyclonal
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Alexa Fluor 647
• Formulation: Purified rabbit polyclonal antibody conjugate in PBS with 1.5% BSA and 0.05% Sodium Azide.
• Gene: ACTA1, ACTA, CFTD, NEM3, MPFD
• Gene Accession No.: P68133
• Host Species: Rabbit
• Immunogen: KLH-conjugated linear peptide corresponding to the N-terminal end sequence of mature skeletal muscle alpha-actin (ACTA1).
• Purification Method: Affinity Purified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Cell Structure
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_001091
• Test Specificity: This Alexa Fluor™ 647 conjugate was prepared from purified rabbit polyclonal antibody (Mfr. No. ABT1485) raised against skeletal muscle alpha-actin (ACTA1) N-terminal end sequence that shares 80% sequence identity in the corresponding region with cardiac muscle alpha-actin (ACTC1), smooth muscle alpha-actin (ACTA2), and cytoplasmic gamma-actin (ACTG1). Immunogen sequence is not found in smooth muscle gamma-actin (ACTG2, a.k.a. ACTA3) and cytoplasmic beta-actin (ACTB).
• Target Species: Human
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Form: Purified

For Research Use Only. Not for use in diagnostic procedures.