Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
Learn More
MilliporeSigma™ ADA3, Mouse, Unlabeled, Clone: 5C9/C8,
Mouse Monoclonal Antibody
Supplier: MilliporeSigma™ MABE1057
Description
Transcriptional adapter 3 (UniProt O75528; also known as ADA3 homolog, ADA3-like protein, Alteration/deficiency in activation 3, hADA3, STAF54, Transcriptional adapter 3-like) is encoded by the TADA3 (also known as ADA3, TADA3L) gene (Gene ID 10474) in human. Alteration/deficiency in activation 3 (ADA3) is a component of several transcriptional co-activator and histone acetyltransferase (HAT) complexes and plays a critical role in in cell cycle regulation. Ada3 deletion in mice is embryonic lethal, and Ada3-deficient mouse embryonic fibroblasts (MEFs) exhiit a severe proliferation defect, dramatic changes in global histone acetylation, mitotic defects, as well as a delay in G2/M-to-G1 and G1-to-S transition. ADA3 also plays a role in genomic stability by controlling DNA repair checkpoints. ChIP-seq analsis reveals that ADA3 is significantly associated with human centromere regions across most chromosomes. In addition, ADA3 is found associated with CENP-B throughout all phases of the cell cycle, and CENP-B centromere binding decreased upon ADA3 knockdown. Wild-type human ADA3, but not CENP-B-binding deficient ADA3 mutant, prevented cell proliferation defects in MEFs following endogenous mouse ADA3 knockown. ADA3 overexpression and mislocalization correlates with poor prognosis in breast cancer patients.
Specifications
ADA3 | |
Monoclonal | |
Please refer to lot specific datasheet. | |
Purified mouse IgG1 in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide. | |
O75528 | |
Full-length recombinant human ADA3. | |
100 μL | |
Epigenetics & Nuclear Function | |
NP_006345 | |
Human | |
Purified |
Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot | |
5C9/C8 | |
Unconjugated | |
TADA3, ADA3, TADA3L | |
Mouse | |
Protein G Purified | |
RUO | |
Primary | |
Clone 5C9/C8 specifically detected Cre recombinase expression-induced ADA3 downregulation in Ada3FL/FL MEFs. Clone 5C9/C8 immunostained the nuclei of untransfected, but not ADA3 shRNA-transfected, 76N-TERT human mammary epithelial cells (Mohibi, S., et al. (2015). J. Biol. Chem. 290(47):28299-28310; Mohibi, S., et al. (2012). J. Biol. Chem. 287(35):29442-29456). | |
Stable for 1 year at 2°-8°C from date of receipt. | |
IgG1 κ |
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
For Research Use Only. Not for use in diagnostic procedures.
Spot an opportunity for improvement?Share a Content Correction