Invitrogen™ ADAM10 Monoclonal Antibody (139712)

Rat Monoclonal Antibody

Supplier:  Invitrogen™ MA523867

Catalog No. PIMA523867

Description

In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) rhADAM10 is observed and no cross-reactivity with rhADAM8, recombinant mouse (rm) ADAM9, rmADAM15, rhBACE, or rhTACE is observed. Reconstitute at 0.5 mg/mL in sterile PBS.

Proinflammatory cytokine tumor necrosis factor-a (TNF-a) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-a, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-a, notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively. ADAM10 mRNA is expressed in a variety of human and bovine tissues.

Specifications

• Antigen: ADAM10
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 5% trehalose and No Preservative
• Gene Accession No.: O35598
• Gene Symbols: Adam10
• Immunogen: Sf 21-derived recombinant mouse ADAM10 Thr215-Glu673.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Flow Cytometry, Western Blot
• Clone: 139712
• Conjugate: Unconjugated
• Gene: Adam10
• Gene Alias: 1700031C13Rik; a disintegrin and metallopeptidase domain 10; a disintegrin and metalloprotease domain (ADAM) 10; a disintegrin and metalloprotease domain 10; a disintegrin and metalloproteinase; AD10; AD18; ADAM; ADAM 10; ADAM metallopeptidase domain 10; ADAM10; ADAMs; CD156c; CDw156; Disintegrin and metalloproteinase domain-containing protein 10; HsT18717; KUZ; kuzbanian; kuzbanian protein homolog; MADM; Mammalian disintegrin-metalloprotease; metalloendopeptidases; metalloproteinase; myelin-associated metalloproteinase; RAK
• Host Species: Rat
• Purification Method: Protein A/G
• Regulatory Status: RUO
• Gene ID (Entrez): 11487
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized