Invitrogen™ Adipolean variant Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 500P205BT500UG

Catalog No. 50-271-8612

Description

AA Sequence of recombinant protein: PGAEGPRGFP GIQGRKGEPG EGAYVYRSAF SVGLETYVTI PNMPIRFTKI FYNQQNHYDG STGKFHCNIP GLYYFAYHIT VYMKDVKVSL FKKDKAMLFT YDQYQENNVD QASGSVLLHL EVGDQVWLQV YGEGERNGLY ADNDNDSTFT GFLLYHDTN. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human gAcrp30/Adipolean Variant. Anti-Human gAcrp30/Adipolean Variant specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hAdipolean Variant by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Adipolean Variant (500-P205) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hAdipolean Variant. Western Blot: To detect Human Adipolean Variant by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Adipolean is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P205BT-1MG will be provided as 2 x 500 μg

The gAcrp30 variant is a naturally occurring globular protein obtained by proteolytic processing of adiponectin. Adiponectin is produced and secreted exclusively by adipocytes, and is a relatively abundant plasma protein, accounting for up to 0.05% of total serum protein. Like Adiponectin, Acrp30 is capable of decreasing hyperglycemia and reversing insulin resistance. Additionally, gAcrp30 has been shown to be an important factor in promoting fat loss by signaling muscle to absorb and burn Free-Fatty Acids (FFAs). The signaling receptors for adiponectin and gAcrp30 have recently been identified and named AdipoR1 and AdipoR2. AdipoR2 is predominantly expressed in the liver. This naturally occurring variant of human gAcrp30/Adipolean is an 18.1 kDa protein, containing 14 extra amino acids extra at the N-terminus of human gAcrp30/Adipolean.

Specifications

• Antigen: Adipolean variant
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: ADIPOQ
• Gene Alias: gAcrp30
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 9370
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: Q15848
• Gene Symbols: ADIPOQ
• Immunogen: E.coli-derived Recombinant Human gAcrp30/Adipolean Variant.
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG