MilliporeSigma™ Adiponectin, Rabbit, Unlabeled, Polyclonal,

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ ABS516

Catalog No. ABS516MI

Description

Adiponectin (UniProt Q8K3R4; also known as 30kDa adipocyte complement-related protein, ACRP30, Adipocyte complement-related 30kDa protein, Adipocyte, C1q and collagen domain-containing protein, Adipose most abundant gene transcript 1 protein, apM-1, Gelatin-binding protein) is encoded by the Adipoq (also known as Acdc, Acrp30) gene (Gene ID 246253) in rat. Adipokines are adipose tissue-secreted factors involved in vascular function and metabolic disease. While most adipokines are proinflammatory and directly responsible for the development of the chronic inflammatory state in obesity, adiponectin is an adipokine with anti-inflammatory and insulin-sensitizing properties. Adiponectin mediates it biological activity via adiponection receptor protein 1 and 2 (AdipoR1 and AdipoR2). Both AdipoR1 and AdipoR2 are reported to be ubiquitously expressed with the highest expression in skeletal muscle and liver, respectively. Circulating adiponectin levels are reduced in obesity and type II diabetes, whereas weight loss elevates serum adiponectin. Likewise, adiponectin overexpression is reported to alleviate metabolic dysfunction independent of weight loss in both genetic and diet-induced animal models of obesity. Aadiponectin deficiency is associated with various clinical cardiovascular disorders including myocardial infarction, peripheral artery disease, and endothelial dysfunction. Adiponectin is produced with a signal peptide sequence (a.a. 1-18 in human and rat sequence; a.a. 1-17 in mouse), which is removed postranslationally to yield the mature protein with a collagen-like domain (a.a. 42-107 in human and rat, a.a. 45-110 in mouse) and a C1q domain (a.a. 108-244 in human and rat, a.a. 111-247 in mouse).

Specifications

• Antigen: Adiponectin
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Adipoq, Acdc, Acrp30
• Host Species: Rabbit
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This rabbit polyclonal antibody was raised against rat adiponectin collagen-like domain and validated for target band detection in both rat and mouse serum samples by Western blotting. Human and Rat adiponectins share 82% sequence identity in the collagen-like domain.
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.

• Applications: Western Blot
• Concentration: Please refer to lot specific datasheet.
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
• Gene Accession No.: Q8K3R4
• Immunogen: GST-tagged recombinant rat adiponectin collagen-like domain.
• Quantity: 100 μg
• Research Discipline: Signaling
• Gene ID (Entrez): NP_653345
• Target Species: Mouse

For Research Use Only. Not for use in diagnostic procedures.