Invitrogen™ Aequorea victoria GFP His-tag Recombinant Protein

Description

Recombinant Protein

Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

Specifications

• For Use With (Application): Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Formulation: PBS with 0.01% Tween 80, 5% mannitol, 5% trehalose and no preservative; pH 7.4
• Molecular Weight (g/mol): 28.7 kDa
• Name: Aequorea victoria GFP His-tag
• Quantity: 50 μg
• Source: E. coli
• Regulatory Status: RUO
• Gene Alias: GFP; GFP tag; GFP2; green fluorescence; green fluorescent; Turbo eGFP
• Common Name: GFP
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Aequorea victoria GFP, amino acids Ser2-Lys238 (Accession # AAB65663) with an N-terminal His-tag
• Content And Storage: -20°Cor to +80°C if preferred
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥90 % as determined by SDS-PAGE. ≥85 % as determined by SEC-HPLC.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov