Invitrogen™ AES Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5121149

Catalog No. PIPA5121149

Description

Positive test controls include: A-549, HeLa, U-251MG, Mouse liver. The target is usually found in the following locations: Nucleus. Immunogen sequence: MCHKNGFPQE GGITAAFLQK RKLRLSKNHR PARAKVTEHV RGTRPGRATA GPAASTRAAG SLFFDRWGNR GPAGCRGSSH LPQQLKFTTS DSCDRIKDEF QLLQAQYHSL KLECDKLASE KSEMQRHYVM YYEMSYGLNI EMHKQAEIVK RLNGICAQVL PYLSQEHQQQ VLGAIERAKQ VTAPELNSII RQQLQAHQLS QLQALALPLT PLPVGLQPPS LPAVSAGTGL LSLSALGSQA HLSKEDKNGH DGDTHQEDDG EKSD.

Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.

Specifications

• Antigen: AES
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: TLE5
• Gene Alias: Aes; AES-1; AES-2; AL024115; Amino enhancer of split; amino-terminal enhancer of split; Esp1; gp130-associated protein GAM; Grg; Grg5; Grg-5; Groucho-related protein 5; Protein ESP1; Protein GRG; related to Drosophila groucho; R-esp1; TLE family member 5; TLE family member 5, transcriptional modulator; TLE family member 5; amino-terminal enhancer of split; TLE5
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 14797, 166
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 8.47 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: P63002, Q08117
• Gene Symbols: AES, TLE5
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human AES (NP_945320.1).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG