Invitrogen™ AES Polyclonal Antibody

Catalog No.	Quantity
PIPA595534	100 μg

Catalog No. PIPA595534 Supplier Invitrogen™ Supplier No. PA595534

Description

Rabbit Polyclonal Antibody

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: human U-87MG whole cell. IHC: human placenta tissue. ICC/IF: U20S cell. Flow: U87 cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.

Specifications

• Antigen: AES
• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 500 μg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 4mg trehalose and 0.05mg sodium azide
• Gene: TLE5
• Gene Accession No.: Q08117
• Gene Alias: Aes; AES-1; AES-2; AL024115; Amino enhancer of split; amino-terminal enhancer of split; Esp1; gp130-associated protein GAM; Grg; Grg5; Grg-5; Groucho-related protein 5; Protein ESP1; Protein GRG; related to Drosophila groucho; R-esp1; TLE family member 5; TLE family member 5, transcriptional modulator; TLE family member 5; amino-terminal enhancer of split; TLE5
• Gene Symbols: AES, TLE5
• Host Species: Rabbit
• Immunogen: E.coli-derived human AES recombinant protein (Position: Y69-L138).
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 166
• Target Species: Human
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG