Invitrogen™ AF9 Polyclonal Antibody

Catalog No.	Quantity
PIPA513174	400 μL

Catalog No. PIPA513174 Supplier Invitrogen™ Supplier No. PA513174

Description

Rabbit Polyclonal Antibody

AF9 Polyclonal Antibody for Western Blot

The human AF9 gene is one of the most common fusion partner genes with the ALL1 gene at 11q23 (also called MLL), resulting in the t(9;11)(p22;q23). The AF9 gene is more than 100 kb, and 2 patient breakpoint cluster regions (BCRs) have been identified; BCR1 is within intron 4, previously called site A, whereas BCR2 or site B spans introns 7 and 8. Several different structural elements have been identified in AF9, including a colocalizing in vivo DNA topo II cleavage site and an in vitro DNase I hypersensitive (DNase 1 HS) site in intron 7 in BCR2. Reversibility experiments demonstrated a religation of the topo II cleavage sites. In addition, 2 scaffold associated regions (SARs) are located centromeric to the topo II and DNase I HS cleavage sites and border breakpoint regions in 2 leukemic cells lines: SAR1 is located in intron 4, whereas SAR2 encompasses parts of exons 5-7. The patient breakpoint regions of AF9 share the same structural elements as the MLL BCR. A DNA breakage and repair model for nonhomologous recombination between MLL and its partner genes, particularly AF9, has been proposed.

Specifications

• Antigen: AF9
• Applications: Western Blot
• Classification: Polyclonal
• Concentration: 2 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.09% sodium azide; pH 7.4
• Gene: MLLT3
• Gene Accession No.: A2AM29, P42568
• Gene Alias: 2210011H10Rik; 2610012I03Rik; 3830408D16Rik; Af9; Af-9; AF-9 protein; ALL1-fused gene from chromosome 9 protein; D4Ertd321e; MLLT3; MLLT3, super elongation complex subunit; myeloid/lymphoid or mixed lineage-leukemia translocation to 3 homolog; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocated to, 3; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3; myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein; Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein homolog; myeloid/lymphoid or mixed-lineage leukemia, translocated to, 3; myeloid/lymphoid or mixed-lineage leukemia,translocated to, 3 (trithorax homolog, Drosophila); myeloid/lymphoid or mixed-lineage leukemia; translocated to 3; myeloid/lymphoid or mixed-lineage leukemia; translocated to, 3; protein AF-9; translocated to, 3; YEATS domain-containing protein 3; YEATS3
• Gene Symbols: MLLT3
• Host Species: Rabbit
• Immunogen: KLH conjugated synthetic peptide between 407-438 amino acids from the central region of human AF9 (MLLT3).
• Purification Method: Ammonium sulfate precipitation, Size-exclusion - Dialysis
• Quantity: 400 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 4300, 70122
• Target Species: Human, Mouse
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG