Invitrogen™ AFAP1L2 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA558134

Catalog No. PIPA558134

Description

Immunogen sequence: SPTLSCLDNA TEALPADSGP GPTPDEPCIK CPENLGEQQL ESLEPEDPSL RITTVKIQTE QQRISFPPSC PDAVVATPPG ASPPVKD Highest antigen sequence identity to the following orthologs: Mouse - 47%, Rat - 49%.

AFAP1L2, also known as XB130, is structurally similar to actin-filament-associated protein (AFAP), containing several SH2- and SH3-binding motifs, two pleckstrin homology domains, a coiled-coil region, and many potential phosphorylation sites. It interacts with and is phosphorylated by c-Src tyrosine kinase. Suppression of AFAP1L2 via siRNA reduced Src activity, IL-8 production, EGF-induced phosphorylation of Akt and GSK3beta, and altered cell cycles in human lung epithelial cells suggesting that AFAP1L2 plays a role as an adaptor in the regulation of Src signal transduction and multiple cellular functions. Recent experiments have shown that AFAP1L2 is highly expressed in thyroid and is the substrate RET/PTC kinase, a thyroid-specific kinase that plays a pathogenic role in papillary thyroid cancer. Down-regulation of AFAP1L2 in these cancer cells reduced Akt activity, inhibiting cell-cycle progression and cancer cell survival in suspension, indicating that AFAP1L2 may be a valuable target in thyroid cancer therapy. At least four isoforms of AFAP1L2 are known to exist.

Specifications

• Antigen: AFAP1L2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: AFAP1L2
• Gene Alias: actin filament associated protein 1 like 2; actin filament associated protein 1-like 2; actin filament-associated protein 1-like 2; Afap1l2; AFAP1-like protein 2; AU041783; C86904; CTB-1144G6.4; CTB-1144G6.6; KIAA1914; mKIAA1914; phosphatidylinositol 3-kinase-associated protein; RGD1310668; XB130
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 84632
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Immunocytochemistry
• Concentration: 0.1 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q8N4X5
• Gene Symbols: AFAP1L2
• Immunogen: Recombinant protein corresponding to Human AFAP1L2. Recombinant protein control fragment (Product #RP-97174).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG