Invitrogen™ Aggrecan Recombinant Rabbit Monoclonal Antibody (JA40-23)

Catalog No.	Quantity
PIMA532695	100 μL

Catalog No. PIMA532695 Supplier Invitrogen™ Supplier No. MA532695

Description

Rabbit Recombinant Monoclonal Antibody

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Aggrecan also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1 is a protein that is encoded by the ACAN gene. The encoded protein is an integral part of the extracellular matrix in cartilaginous tissue and it withstands compression in cartilage. The human form of the protein is 2316 amino acids long (approximately 250 kDa) and can be expressed in multiple isoforms due to alternative splicing. Aggrecan exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate chains are attached to an extended protein core. The synthesis and degradation of aggrecan are being investigated for their roles in cartilage deterioration during joint injury, disease, and aging. It contains three interglobular domains (IGDs), G1, G2, and G3 that are involved in aggregation, hyaluronan binding, cell adhesion, and chondrocyte apoptosis. Aggrecan fragments from articular cartilage are released into the synovial fluid at all stages of human osteoarthritis as a result of cleavage by aggrecanase and MMP activity. Antibodies BC-3 and BC-4 can be used to detect these neo epitopes of Aggrecanase (ARGSVXXX) and MMP (XXXVDIPEN), respectively.

Specifications

• Antigen: Aggrecan
• Applications: Immunohistochemistry (Paraffin)
• Classification: Recombinant Monoclonal
• Clone: JA40-23
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: TBS with 0.05% BSA, 40% Glycerol and 0.05% sodium azide; pH 7.4
• Gene: ACAN
• Gene Accession No.: P07897, P16112
• Gene Alias: ACAN; Agc; Agc1; AGCAN; aggrecan; aggrecan 1; aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122); aggrecan core protein; Aggrecan core protein 2; aggrecan CS2 domain; aggrecan epidermal growth factor-like domain-1; aggrecan G2 domain; aggrecan interglobular domain; aggrecan keratan sulfate domain; aggrecan, structural proteoglycan of cartilage; articular cartilage aggrecan precursor; b2b183Clo; cartilage aggregating proteoglycan; cartilage-specific proteoglycan core protein; Chondroitin sulfate proteoglycan 1; chondroitin sulfate proteoglycan core protein 1; cmd; CSPCP; CSPG1; CSPGCP; high density cartilage proteoglycan; large aggregating cartilage proteoglycan core protein; large aggregating proteoglycan; large aggregating proteoglycan, antigen; MSK16; SEDK
• Gene Symbols: ACAN
• Host Species: Rabbit
• Immunogen: Recombinant protein within Human Aggrecan aa 742-881.
• Purification Method: Protein A
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 176, 58968
• Target Species: Human, Rat
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG