Invitrogen™ Agrin Monoclonal Antibody (AGR 131)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA125500

Catalog No. MA125500

Description

This antibody detects all N1 isoforms, but fails to detect the N4 protein. Also, the antibody will detect the X, Y, and Z splice variants.

Agrin is a molecule that resides in the basal lamina of muscle cells and directs key events in post synaptic differentiation. Most notably, Agrin is responsible for the clustering of acetylcholine receptors (AChRs) on the cell surface and their localization to the neuromuscular junction. Several Agrin variants have been identified which arise from alternative mRNA splicings. Agrin splice forms having inserts at two sites in the carboxy terminus designated y and z display a high affinity for AChRs, while splice forms lacking these inserts associate with AChRs weakly. Muscle alpha-dystroglycan has been postulated to be the receptor for the clustering activity of agrin; however, this is a point of contention. Tyrosine phosphorylation has been implicated as a required early step in AChR aggregation. Interestingly, a unique receptor tyrosine kinase, designated MuSK, has been discovered that interacts with Agrin and is specifically localized to developing muscle.

Specifications

• Antigen: Agrin
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol, 0.1mM PMSF and no preservative; pH 7.2
• Gene Accession No.: A2ASQ1, P25304
• Gene Symbols: Agrn
• Immunogen: Recombinant rat agrin, C-terminal construct.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunohistochemistry (Frozen), Radioimmune Assays (RIA), Western Blot, Immunocytochemistry
• Clone: AGR 131
• Conjugate: Unconjugated
• Gene: Agrn
• Gene Alias: AGR; Agrin; Agrin C-terminal 110 kDa subunit; Agrin C-terminal 22 kDa fragment; Agrin C-terminal 90 kDa fragment; Agrin N-terminal 110 kDa subunit; agrin proteoglycan; Agrn; C22; C90; CMS8; CMSPPD; nmf380; RP23-139J18.2
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 11603, 25592
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov