Invitrogen™ Agrin Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA537121

Catalog No. PIPA537121

Description

This antibody detects endogenous protein at a molecular weight of 217 kDa. Purity is >95% by SDS-PAGE.

Agrin is a molecule that resides in the basal lamina of muscle cells and directs key events in post synaptic differentiation. Most notably, Agrin is responsible for the clustering of acetylcholine receptors (AChRs) on the cell surface and their localization to the neuromuscular junction. Several Agrin variants have been identified which arise from alternative mRNA splicings. Agrin splice forms having inserts at two sites in the carboxy terminus designated y and z display a high affinity for AChRs, while splice forms lacking these inserts associate with AChRs weakly. Muscle alpha-dystroglycan has been postulated to be the receptor for the clustering activity of agrin; however, this is a point of contention. Tyrosine phosphorylation has been implicated as a required early step in AChR aggregation. Interestingly, a unique receptor tyrosine kinase, designated MuSK, has been discovered that interacts with Agrin and is specifically localized to developing muscle.

Specifications

• Antigen: Agrin
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Agrn
• Gene Alias: AGR; Agrin; Agrin C-terminal 110 kDa subunit; Agrin C-terminal 22 kDa fragment; Agrin C-terminal 90 kDa fragment; Agrin N-terminal 110 kDa subunit; agrin proteoglycan; Agrn; C22; C90; CMS8; CMSPPD; nmf380; RP23-139J18.2
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 11603, 25592, 375790
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: A2ASQ1, O00468, P25304
• Gene Symbols: Agrn
• Immunogen: A synthetic peptide corresponding to residues in human AGRN.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG