MilliporeSigma™ AGS3/GPSM1, Rabbit, Unlabeled, Polyclonal,

Catalog No.	Quantity	Conjugate
ABS1531MI	100 μL	Unconjugated

Catalog No. ABS1531MI Supplier MilliporeSigma™ Supplier No. ABS1531

Description

Rabbit Polyclonal Antibody

G-protein-signaling modulator 1 (UniProt Q9R080; also known as Activator of G-protein signaling 3) is encoded by the Gpsm1 (also known as Ags3) gene (Gene ID 246254) in rat species. AGS3/GPSM1 is an evolutionarily conserved protein with four G-protein regulatory (GPR) GoLoco motifs (a.a. 493-515, 546-568, 594-616, 628-650 of murine GPSM1) and functions as a guanine nucleotide dissociation inhibitor (GDI). GPSM1 increases heteromeric polycystin-1/polycystin-2 ion channel activity via Gβγ subunits. GPSM1 is involved in regulating mitotic spindle orientation, cell polarity, and adenylyl cyclase activity. GPSM1 plays a role in renal epithelial cell repair following renal injury and GPSM1 induction in polycystic kidney disease (PKD) and may be a critical modulator of the renal cystogenic process.

Specifications

• Antigen: AGS3/GPSM1
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Classification: Polyclonal
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Rabbit polyclonal antibody serum with 0.05% Sodium Azide.
• Gene: Gpsm1, Ags3
• Gene Accession No.: Q9R080
• Host Species: Rabbit
• Immunogen: Linear peptide corresponding to a sequence within the STK11/LKB1-interacting region of rat AGS3/GPSM1.
• Purification Method: Unpurified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Signaling
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_653346
• Test Specificity: This polyclonal antiserum immunostained predominantly renal collecting ducts of postnatal P12 ADPKD mice. Immunogen peptide blocking eliminated antibody stianing (Kwon, M., et al. (2012). Proc. Natl. Acad. Sci. U. S. A. 109(52):21462-21467). This antiserum targets a STK11/LKB1-interacting region sequence present in rat AGS3/GPSM1 isoform 1 and isoform 4 (isoform Long), 28% present in isoform 3 (Short-2), but completely absent from isoforms 2 (Short-1) reported by UniProt (Q9R080). Target sequence is also present in mouse isoforms 1, 3, 4, and 5, but absent from isoform 2 ( (UniProt Q6IR34).
• Target Species: Mouse
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Form: Serum

For Research Use Only. Not for use in diagnostic procedures.