Invitrogen™ AHR Recombinant Rabbit Monoclonal Antibody (1B10E5)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA542843

Catalog No. PIMA542843

Description

Positive test controls include: HepG2, PC-3. Immunogen sequence: LEQQQQHHQK QVVVEPQQQL CQKMKHMQVN GMFENWNSNQ FVPFNCPQQD PQQYNVFTDL HGISQEFPYK SEMDSMPYTQ NFISCNQPVL PQHSKCTELD YPMGSFEPSP YPTTSSLEDF VTCLQLPENQ KHGLNPQSAI ITPQTCYAGA VSMYQCQPEP QHTHVGQMQY NPVLPGQQAF LNKFQNGVLN ETYPAELNNI NNTQTTTHLQ PLHHPSEARP FPDLTSSGFL.

AHR (Ah Receptor) belongs to a family of proteins comprised of its dimerization partner ARNT (HIF-1 Beta) and the Drosophila proteins PER and SIM. AHR contains an N-terminal sequence of approximately 200 amino acids termed the PAS domain. AHR, found in a variety of tissues, binds to a specific DNA enhancer sequence and initiates transcription of the mRNA for the cytochrome P-450 (CYPIA1) gene. The gene for AHR encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. AHR has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450, and its ligands included a variety of aromatic hydrocarbons. AHR is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for AHR. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form, and this transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). Diseases associated with AHR include eosinophilic fasciitis and seborrheic dermatitis.

Specifications

• Antigen: AHR
• Classification: Recombinant Monoclonal
• Concentration: 1.1 mg/mL
• Formulation: PBS with 0.05% BSA, 50% glycerol and 0.05% ProClin 300; pH 7.3
• Gene Accession No.: P35869
• Gene Symbols: AHR
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 619-848 of human AHR (NP_0016121).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

• Applications: ELISA, Western Blot
• Clone: 1B10E5
• Conjugate: Unconjugated
• Gene: AHR
• Gene Alias: Ah; Ah receptor; Ahh; Ahr; Ahre; AH-receptor; aromatic hydrocarbon receptor; aryl hydrocarbon receptor; aryl-hydrocarbon receptor; bHLHe76; Class E basic helix-loop-helix protein 76; dioxin receptor; In
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 196
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

Safety and Handling

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