Invitrogen™ AIF Polyclonal Antibody

Catalog No.	Quantity
PIPA519960	100 μg

Catalog No. PIPA519960 Supplier Invitrogen™ Supplier No. PA519960

Description

Rabbit Polyclonal Antibody

In Western blot applications, this antibody detects a band at ~67kDa. A suggested positive control is K562 cell lysate. PA5-19960 can be used with blocking peptide PEP-0085.

Apoptosis Inducing Factor (AIF) causes chromatin condensation and DNA fragmentation. AIF was recently identified, and cloned. Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed. Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants of AIF and a related pseudogene has been identified on chromosome 10.

Specifications

• Antigen: AIF
• Applications: Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.02% sodium azide
• Gene: AIFM1
• Gene Accession No.: O95831, Q9JM53, Q9Z0X1
• Gene Alias: AG1; AGR1; Aif; AIFM1; AIFsh2; apoptosis inducing factor mitochondria associated 1; apoptosis inducing factor, mitochondria associated 1; apoptosis-inducing factor 1, mitochondrial; apoptosis-inducing factor, mitochondrion-associated 1; apoptosis-inducing factor, mitochondrion-associated, 1; CMT2D; CMTX4; COWCK; COXPD6; DFNX5; ER protein 18; ER protein 19; ERP18; ERP19; hAG-1; harlequin; Hq; hTLP19; MGC111425; mitochondrial apoptosis-inducing factor 1; NADMR; NAMSD; pcd8; PDCD8; PDIA16; programmed cell death 8 (apoptosis-inducing factor); programmed cell death protein 8; RP3-438D16.2; striatal apoptosis-inducing factor; testicular secretory protein Li 4; TLP19; UNQ713/PRO1376
• Gene Symbols: AIFM1
• Host Species: Rabbit
• Immunogen: A peptide corresponding to amino acids 593 to 606 of human AIF.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 26926, 83533, 9131
• Target Species: Human, Mouse, Rat
• Content And Storage: Maintain refrigerated at 2°C to 8°C for up to 3 months. For long term storage store at -20°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG