AKT1 Mouse anti-Human, Mouse, Rat, APC, Clone: 5E5.F5.D7, Rockland™

Catalog No.	Quantity
50-199-0726	50 μg

Catalog No. 50-199-0726 Supplier Rockland Immunochemicals Supplier No. 200326J34

Description

Mouse Monoclonal Antibody

Store vial at 4°C prior to restoration. Restore with deionized water (or equivalent). This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis. Anti-AKT1 antibody is directed against human AKT1. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected.

AKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

Specifications

• Antigen: AKT1
• Applications: Dot Blot, ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
• Classification: Monoclonal
• Clone: 5E5.F5.D7
• Concentration: 1 mg/mL
• Conjugate: APC
• Formulation: 0.02M potassium phosphate with 0.5M NaCl, 10 mg/mL BSA and no preservative; pH 7.2
• Gene: AKT1
• Gene Accession No.: P31749, P31750, P47196
• Gene Alias: Akt; Akt kinase; AKT serine/threonine kinase 1; AKT/PKB; AKT1; AKT1 kinase; AKT1m; Akt1m protein; Akt1-PA; Akt1-PB; Akt1-PC; Akt1-PD; Akt1-PE; AKT2; AKT3; CAKT; C-AKT; CG4006; CG4006 gene product from transcript CG4006-RE; CG4006-PA; CG4006-PB; CG4006-PC; CG4006-PD; CG4006-PE; CWS6; DAkt; D-Akt; dAkt kinase; dAKT/dPKB; dAkt/PKB; DAkt1; DAKT1/PKB; Dmel\CG4006; Dmel_CG4006; dPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; kinase Akt1; l(3)04226; l(3)89Bq; MGC99656; murine thymoma viral (v-akt) oncogene homolog 1; pAkt; p-Akt; Phospho-AKT; Phospho-PKB; PKB; PKB Akt; PKB alpha; PKB beta; PKB gamma; PKB/AKT; PKB/dAKT; PKBalpha; PKB-ALPHA; PKBG; PRKBA; Protein kinase B; Protein kinase B alpha; protein kinase B-alpha; Proto-oncogene c-Akt; Rac; rac protein kinase alpha; RAC protein kinase alpha RAC-PK alpha; RAC serine/threonine-protein kinase; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RacPK; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; related to PKA to PKC protein kinases; related to the A and C kinases; serine/threonine protein kinase; serine-threonine protein kinase; STK-2; Thymoma viral proto-oncogene; thymoma viral proto-oncogene 1; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1
• Gene Symbols: AKT1
• Host Species: Mouse
• Immunogen: Anti-AKT1 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1 protein followed by monoclonal development.
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 11651, 207, 24185
• Target Species: Human, Mouse, Rat
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG2a κ