Invitrogen™ AKT1 (PH domain) Recombinant Rabbit Monoclonal Antibody (RM316)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA533070

Catalog No. PIMA533070

Description

Specificity: reacts to human AKT (PH domain). It may also react to mouse or rat AKT, as predicted by immunogen homology. Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.

Specifications

• Antigen: AKT1 (PH domain)
• Classification: Recombinant Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 1% BSA, 50% glycerol and 0.09% sodium azide
• Gene Accession No.: P31749
• Gene Symbols: AKT1
• Immunogen: A peptide corresponding to the PH domain of human AKT.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Clone: RM316
• Conjugate: Unconjugated
• Gene: AKT1
• Gene Alias: AKT; AKT serine/threonine kinase 1; AKT1; AKT1m; CWS6; PKB; PKB alpha; PKB-ALPHA; PRKBA; Protein kinase B; Protein kinase B alpha; proto-oncogene c-Akt; RAC; rac protein kinase alpha; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; serine-threonine protein kinase; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 207
• Content And Storage: -20°C
• Form: Liquid