ALAS1 Mouse anti-Human, Clone: OTI1C5, lyophilized, TrueMAB™

Catalog No.	Quantity
50-166-8672	100 μg

Catalog No. 50-166-8672 Supplier OriGene Supplier No. CF808177

Description

Mouse Monoclonal Antibody

Reconstitute with PBS (pH 7.3) and recommend to perform another round of desalting process using Product No. 7KMWCO

ALAS-1 is a nuclear-encoded mitochondrial matrix enzyme which catalyzes the condensation of glycine with succinyl-CoA to form delta-amino levulinate, CO2 and CoA. It regulates the first and rate-limiting step of heme biosynthetic pathway. It is one of the two isoforms of ALAS and is a pyridoxal-5′ phosphate dependant housekeeping enzyme. It is ubiquitously expressed and is responsible of providing heme for cytochromes and other hemoproteins. ALAS1 in liver undergoes negative feedback regulation by heme. Transcription of ALAS1 gene is stimulated by cAMP and respiratory uncoupling whereas Phorbol esters and insulin repress ALAS1 gene expression. ALAS1 is suggested to be involved in reciprocal regulation of Heme biosynthesis and circadian clock suggesting a potential target for treatment of circadian disorders. ALAS1 gene expression is down regulated in Acute Liver failure resulting in altered heme metabolism and liver function.

Specifications

• Antigen: ALAS1
• Applications: Western Blot
• Classification: Monoclonal
• Clone: OTI1C5
• Conjugate: Unconjugated
• Formulation: PBS with 8% trehalose and no preservative; pH 7.3
• Gene: ALAS1
• Gene Accession No.: P13196
• Gene Alias: ALAS, ALAS-H, ALAS3, ALASH, MIG4
• Gene Symbols: ALAS1
• Host Species: Mouse
• Immunogen: Human recombit protein fragment corresponding to amino acids 57-308 of human ALAS1 produced in E.coli.
• Purification Method: Affinity Chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 211
• Target Species: Human
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG1