Invitrogen™ ALAS1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA529966

Catalog No. PIPA529966

Description

Recommended positive controls: Raji. Predicted reactivity: Mouse (87%), Rat (85%), Bovine (85%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

ALAS-1 is a nuclear-encoded mitochondrial matrix enzyme which catalyzes the condensation of glycine with succinyl-CoA to form delta-amino levulinate, CO2 and CoA. It regulates the first and rate-limiting step of heme biosynthetic pathway. It is one of the two isoforms of ALAS and is a pyridoxal-5' phosphate dependant housekeeping enzyme. It is ubiquitously expressed and is responsible of providing heme for cytochromes and other hemoproteins. ALAS1 in liver undergoes negative feedback regulation by heme. Transcription of ALAS1 gene is stimulated by cAMP and respiratory uncoupling whereas Phorbol esters and insulin repress ALAS1 gene expression. ALAS1 is suggested to be involved in reciprocal regulation of Heme biosynthesis and circadian clock suggesting a potential target for treatment of circadian disorders. ALAS1 gene expression is down regulated in Acute Liver failure resulting in altered heme metabolism and liver function.

Specifications

• Antigen: ALAS1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ALAS1
• Gene Alias: 5'-aminolevulinate synthase 1; 5-aminolevulinate synthase 1; 5-aminolevulinate synthase, nonspecific, mitochondrial; 5-aminolevulinic acid synthase 1; ALAS; ALA-S; ALAS1; Alas-1; ALAS3; ALASH; Alas-h; ALAS-N; aminolevulinate synthase H; aminolevulinate, delta-, synthase 1; aminolevulinic acid synthase 1; delta-ALA synthase 1; delta-ALA synthetase; delta-aminolevulinate synthase 1; MIG4; migration-inducing protein 4; OK/SW-cl.121; succinyl-CoA: glycine C-succinyl transferase
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 211
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.71 mg/mL
• Formulation: 0.1M tris glycine with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: P13196
• Gene Symbols: ALAS1
• Immunogen: Recombinant fragment corresponding to a region within amino acids 62 and 304 of Human ALAS-H.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG