Albumin, Bovine, Cohn Fraction V, 98%, Immunoassay Grade, protease and enzyme-free, pH 7.0, Thermo Scientific Chemicals

Albumin bovine/fraction V, CAS # 9048-46-8, also known as bovine serum albumin (BSA), is a serum albumin protein derived from cows.

Supplier:  Thermo Scientific Chemicals J6573130

Catalog No. AAJ6573130

Description

Protease-sensitive Immunoassays such as RIA, EIA, Fluorescent and Chemiluminescent, Protein Standard, Diluent, Protein, Conjugate or Enzyme Stabilizer. Bovine Serum Albumin is applicable for numerous biochemical applications including ELISAs (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry. Frequently used as a blocking reagent; particularly useful with casein-sensitive antibodies, such as phospho-specific antibodies. Also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. Commonly used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA. Hybridization, Selected Cell Culture applications

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• Albumin bovine/fraction V is a serum albumin protein commonly used in laboratory experiments including, enzyme-linked immunosorbent assay (ELISAs), immunoblots and immune-histochemistry or -fluorescence

Applications

• Albumin bovine/fraction V displays blocking activity mainly when casein-sensitive antibodies, including phospho-specific antibodies, are used
• In genetic approaches this compound can be used to increase polymerase chain reactions (PCR) yields from low purity templates
• This compound is useful for stabilizing enzymes during storage and also for enzymatic reactions where the absence of nucleases is crucial
• In in vitro cultures, albumin bovine/fraction V has been applied as a nutrient in cell and microbial cultures especially when serum-free media is used
• It is commonly used as a protein standard to obtain calibration curves for several laboratorial experiments
• Albumin bovine/fraction V is a small protein commonly used in immunohistochemistry to block nonspecific regions. The binding of this compound to nonspecific binding sites increases the probability of the antibodies binds only to the region of interest.

Specifications

• Formulation: Lyophilized powder
• Quantity: 250g
• Storage Requirements: 2° to 8°C
• Product Type: Albumin, Bovine, Cohn Fraction V, 98%, Immunoassay grade, protease and enzyme-free, pH7.0
• Form: Lyophilized

• pH Range: 7.0
• Source: Bovine Serum
• Regulatory Status: RUO
• Recombinant: Native
• Purity or Quality Grade: Immunoassay grade, 98%

Safety and Handling

EINECSNumber : 232-936-2

Recommended Storage : Keep cold

RUO – Research Use Only