MilliporeSigma™ AldeGreen™ ALDH Detection Assay

Description

Live cancer stem cell assay using green-shifted fluorescent dye for ALDH

• Allows concurrent use of other red fluorescent reporters or probes enables flow cytometry-based detection of intracellular Aldehyde Dehydrogenase (ALDH) enzyme activity
• High ALDH activity serves as a universal marker of stem cells, both normal and malignant

Includes:

• • AldeGreen™ Reagent: 1 vial containing 50 μg of AldeGreen™ reagent powder
  • DEAB Reagent: 1 vial containing 1 mL of DEAB (Diethylaminobenzaldehyde) reagent
  • Hydrochloric Acid (2N): 1 vial containing 1.5 mL of 2 Normal hydrochloric acid
  • Dimethylsulphoxide: 1 vial containing 1.5 mL of Dimethylsulphoxide (DMSO)
  • AldeGreen™ Assay Buffer: 4 bottles containing 25 mL of AldeGreen™ Assay Buffer each
  • Verapamil: 4 vials containing 615 μg Verapamil powder each

Specifications

• Content And Storage: Store at 2°C to 8°C, Reconstituted and activated may be stored in aliquots (at -20 °C protected from light for up to 1 year, Not recommended repeated freezing and thawing)
• Detection Method: Fluorescence
• For Use With (Application): Flow Cytometry, Fluorescence Activated Cell Sorting (FACS)
• Emission: 488
• Excitation Wavelength Range: 515 to 545 nm
• Quantity: 1 Kit

For research purposes only