Tocris Bioscience™ ALDH Detection Reagent - BAAA-DA

Catalog No.	Quantity
75-561-00U	100 μg

Catalog No. 75-561-00U Supplier Tocris Bioscience™ Supplier No. 7556/100U

Description

Fluorescent ALDH substrate and detection reagent. Used for: identification of cells with high ALDH activity. Application: flow cytometry, fluorescent-activated cell sorting (FACS)

Key information: ALDH Detection Reagent - BAAA-DA is a fluorescent substrate of aldehyde dehydrogenase (ALDH).

Used for: can be used in flow cytometry to sort cancer stem cells with high ALDH levels, and to isolate human hematopoietic progenitor cells. Can be used with efflux inhibitors such as Verapamil (Cat. No. 0654), Probenecid (Cat. No. 4107) or 2-deoxy-D-glucose (Cat. No. 4515) to block multi-drug resistance efflux.

Application: flow cytometry, fluorescent-activated cell sorting (FACS).

Properties and Photophysical Data: BAAA-DA is converted under acidic conditions to BAAA. BAAA, cell-permeant, is converted by intracellular ALDH into carboxylate ion amino acetate (BAA) and used to identify cells with high ALDH activity. Excitation and emission maxima (λ) are 505 nm and 512 nm, respectively; extinction coefficient = 74,595 M-1cm-1.

Specifications

• Chemical Name or Material: (T-4)-[N-(2,2-Diethoxyethyl)-5-[(3,5-dimethyl-2H-pyrrol-2-ylidene-κN)methyl]-1H-pyrrole-2-propanamidato-κN1]difluoroboron
• CAS: 247069-93-8
• Quantity: 100 μg
• Target: Enzyme Probes and Enzyme Substrates
• Molecular Formula: C₂₀H₂₈BF₂N₃O₃
• Purity: 0.95