Invitrogen™ Aldosterone Competitive ELISA Kit

Description

ELISA

The Aldosterone Competitive ELISA quantitates aldosterone in extracted serum,plasma,urine,dried fecal extracts or cell culture medium.

Principle of the method

The Aldosterone Competitive ELISA Kit research-use-only kit is designed to quantitatively measure aldosterone present in extracted serum and plasma,or in urine,extracted dried fecal samples,and tissue culture media samples. An aldosterone stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into the provided plate coated with a capture antibody. An aldosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to aldosterone to each well. After incubation,the plate is washed and substrate is added. The substrate reacts with the bound aldosterone-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

Aldosterone is a hormone produced by the adrenal glands that helps regulate blood pressure through increasing both the release of potassium and the uptake of water and sodium by the kidneys. It is essential for sodium conservation in the kidney, salivary glands, sweat glands and colon. It plays a central role in the regulation of the plasma sodium (Na+), the extracellular potassium (K+) and arterial blood pressure. It does so mainly by acting on the mineralocorticoid receptors in the distal tubules and collecting ducts of the nephron. It influences the reabsorption of sodium and excretion of potassium (from and into the tubular fluids, respectively) of the kidney, thereby indirectly influencing water retention or loss, blood pressure and blood volume. When dysregulated, aldosterone is pathogenic and contributes to the development and progression of cardiovascular and renal disease. Aldosterone has exactly the opposite function of the atrial natriuretic hormone secreted by the heart.

Specifications

• Assay Range: 3.906 to 4000 pg/mL
• Assay Sensitivity: 4.97 pg/mL
• Description: Aldosterone Competitive ELISA Kit
• Conjugate: HRP
• Product Type: ELISA
• Protein Family: Steroid hormone
• Sample Type: Fecal Extract, Plasma, Serum, Supernatant, Urine
• For Use With (Application): ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
• For Use With (Equipment): Colorimetric Microplate Reader
• Interassay CV: 19.5%
• Intraassay CV: 6.9
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Research Discipline: Reproductive Biology
• Sample Volume: Fecal Extract 100 μL, Plasma 100 μL, Serum 100 μL, Supernatant 100 μL, Urine 25 μL
• Storage Requirements: -20°C
• Target: Aldosterone
• Test Time: 1 hrs 20 mins
• Total Assay Time: 16 hrs 45 mins