Molecular Probes™ Alexa Fluor™ 488 Protein Labeling Kit 

Alexa Fluor™ 488 Protein Labeling Kit

Manufacturer:  Molecular Probes™ A10235

Catalog No. A10235

Description

Molecular Probes™ Protein Labeling Kits provide a convenient means for attaching a fluorescent label (or biotin) to an antibody (or a protein larger than 40 kDa). Conjugates are ideal for multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, FISH, and more. Kits are available in 12 Alexa Fluor™ dye colors, biotin, the hapten Oregon Green™ 488, fluorescein EX, and Texas Red™ dye. Each kit provides the components needed to perform three protein conjugations and purifications.

Important Features of Protein Labeling Kits:

Labeled proteins typically ready to use in 2 hr (~30 min hands-on time)

Designed to label 1 mg of IgG

Simple protocol — react, separate, use

Stabilizing proteins must be removed from the sample before labeling

The Benefits of Alexa Fluor™ Dyes
Compared to traditional dyes, Alexa Fluor™ dyes are brighter, more photostable, and more pH resistant between pH 4 and 10. And generally when using Alexa Fluor™ dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor™ Dyes Spanning the Visible and Infrared Spectrum—Section 1.3.

Learn More About Protein and Antibody Labeling
We offer a wide selection of Molecular Probes™ antibody and protein labeling kits to fit your starting material and your experimental setup. See Antibody Labeling from A to Z or use our Labeling Chemistry Selection Tool for other choices. To learn more about our various kits read Kits for Labeling Proteins and Nucleic Acids—Section 1.2 in the Molecular Probes™ Handbook.

We’ll Make a Custom Antibody Conjugate for You
If you can’t find what you’re looking for in our stocked list, we’ll prepare a custom antibody conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

Specifications

• Content And Storage: Store in refrigerator (2–8°C) and protect from light.
• Excitation/Emission: 495⁄519
• Labeling Scale: 1 mg
• Color: Green
• Label or Dye: Alexa Fluor™ 488

• Detection Method: Fluorescent
• Labeling Method: Conjugation-Based
• Shipping Condition: Room Temperature
• Label Type: Alexa Fluor Dyes
• Product Line: Alexa Fluor™

For Research Use Only. Not for use in diagnostic procedures.