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Molecular Probes™ Alexa Fluor™ 647 Hydrazide Non-distribution product as customer accommodation.

Alexa Fluor™ 647 Hydrazide

Manufacturer:  Molecular Probes™ A20502

Catalog No. A20502


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Description

Description

Alexa Fluor™ 647 Hydrazide is useful as a cell tracer and as a reactive dye for labeling aldehydes or ketones in polysaccharides or glycoproteins. Alexa Fluor™ 647 is a bright, far red fluorescent dye with excitation ideally suited to the 633 nm laser line. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor™ 647 dye is water soluble and pH-insensitive from pH 4 to pH 10. In addition to reactive dye formulations, we offer Alexa Fluor™ 647 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection (learn more).

Detailed information about this AlexaFluor™ hydrazide:

  • Fluorophore label : Alexa Fluor™ 647 dye
  • Reactive group: hydrazide
  • Reactivity: Aldehydes or keytones in polysaccharides or glycoproteins
  • Ex/Em of the conjugate: 649/666 nm
  • Extinction coefficient: 250,000 cm-1M-1
  • Spectrally similar dyes: APC, Cy5
  • Molecular weight: ~1,200

    Cell Tracking and Tracing Applications
    Alexa Fluor™ hydrazides and hydroxlamines are useful as low molecular weight, membrane-impermeant, aldehyde-fixable cell tracers, exhibiting brighter fluorescence and greater photostability than cell tracers derived from other spectrally similar fluorophores. They are easily loaded into cells by microinjection, infusion from patch pipette, or uptake induced by our Influx™ Pinocytic Cell-Loading Reagent. Learn more about cell tracking and tracing.

    Glycoprotein and Polysaccharide Labeling Applications
    The Alexa Fluor™ hydrazides and hydroxlamines are reactive molecules that can be used to add a fluorescent label to biomolecules containing aldehydes or ketones. Aldehydes and ketones can be introduced into polysaccharides and glycoproteins by periodate-mediated oxidation of vicinal diols. Galactose oxidase can also be used to oxidize terminal galactose residues of glycoproteins to aldehydes.

    Hydrazide vs Hydroxylamine
    Hydrazine derivatives react with ketones and aldehydes to yield relatively stable hydrazones. Hydroxylamine derivatives (aminooxy compounds) react with aldehydes and ketones to yield oximes. Oximes are superior to hydrazones with respect to hydrolytic stability. Both hydrazones and oximes can be reduced with sodium borohydride (NaBH4) to further increase the stability of the linkage.

    Learn More About Protein and Antibody Labeling
    We offer a wide selection of Molecular Probes™ antibody and protein labeling kits to fit your starting material and your experimental setup. See our Antibody Labeling kits or use our Labeling Chemistry Selection Tool for other choices. To learn more about our labeling kits, read Kits for Labeling Proteins and Nucleic Acids—Section 1.2 in The Molecular Probes™ Handbook.

    We’ll Make a Custom Conjugate for You
    If you can’t find what you’re looking for in our online catalog, we’ll prepare a custom antibody or protein conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.

    Related Products
    DMSO (dimethylsulfoxide) (D12345)
    Antibody Conjugate Purification Kit for 0.5-1 mg (A33086)
    Antibody Conjugate Purification Kit for 20-50µg (A33087)
    Antibody Conjugate Purification kit for 50-100µg (A33088)
  • Specifications

    Specifications

    Alexa Fluor™ 647 Hydrazide, 1mg, Blue Solid, ∽1, 200 Molecular Weight, Hydrazide Reactive Group, 666nm Emission 649nm Excitation, 86% Purified by HPLC at 647nm, Room Temperature Storage Requirements, Used for Cell Tracing and Tracking and Cellu
    Blue
    Cell tracing and tracking and cellular imaging and labeling chemistry, cell analysis, labeling carboxylic acids, aldehydes and ketones, labeling with fluorophores, cellular imaging, in-vivo imaging, neuronal tracing
    Alexa Fluor™ 647 Hydrazide
    1mg
    649nm
    86% by HPLC
    Solid
    Solid
    Alexa Fluor™ 647
    Room temperature
    ∽1200kDa
    666nm
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