ALK/CD246 Antibody (ALK/1503), Alexa Fluor™ 594, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP254375AF594

Catalog No. NP2437A94

Description

ALK/CD246 Monoclonal specifically detects ALK/CD246 in Human samples. It is validated for Western Blot.

Specifications

• Antigen: ALK/CD246
• Classification: Monoclonal
• Conjugate: Alexa Fluor 594
• Formulation: 50 mM sodium borate with 0.05% sodium azide
• Gene Symbols: ALK
• Immunogen: Recombinant human ALK/CD246 protein fragment (aa1360-1460) (Uniprot: Q9UM73 )
• Quantity: 0.1 mL
• Primary or Secondary: Primary
• Test Specificity: The wild-type anaplastic lymphoma kinase (ALK) protein is a 200kDa transmembrane receptor tyrosine kinase. Its expression is restricted to a few scattered cells in the nervous system (some glial cells and neurons, and a few endothelial cells and pericytes. The hybrid gene,NPM-ALK, created by the t(2;5)(p23;q35) chromosomal translocation encodes part of the nucleolar phosphoprotein, nucleophosmin (NPM), joined to the entire cytoplasmic portion of the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. As a consequence, the ALK gene comes under the control of the NPM promoter, which induces a permanent and ubiquitous transcription of the NPM-ALK hybrid gene, resulting in the production of a 80kDa NPM-ALK chimeric protein. This translocation is found in anaplastic large cell lymphomas (ALCL). Reportedly, expression of ALK indicates a better prognosis. Approximately 5%-10% of non-small cell lung carcinomas also express ALK protein producing a cytoplasmic staining pattern. This monoclonal antibody also reacts with blood vessels that serves as an internal positive control.
• Content And Storage: Store at 4°C in the dark.

• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence
• Clone: ALK/1503
• Dilution: Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence
• Gene Alias: ALK tyrosine kinase receptor, Anaplastic lymphoma kinase, anaplastic lymphoma kinase (Ki-1), anaplastic lymphoma receptor tyrosine kinase, CD246, CD246 antigen, EC 2.7.10.1, NBLST3
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Research Discipline: Phospho Specific, Protein Kinase, Signal Transduction
• Gene ID (Entrez): 238.0
• Target Species: Human
• Isotype: IgG1 κ