Alkaline Phosphatase/ALPP Antibody (ALPP/516), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP24495700	Novus Biologicals NBP2449570.02MG	0.02 mg
NBP24495701	Novus Biologicals NBP2449570.1MG	0.1 mg
NBP24495702	Novus Biologicals NBP2449570.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Frozen), Immunofluorescence

Alkaline Phosphatase/ALPP Monoclonal specifically detects Alkaline Phosphatase/ALPP in Human samples. It is validated for ELISA.

Specifications

• Antigen: Alkaline Phosphatase/ALPP
• Concentration: 0.2 mg/mL
• Applications: Flow Cytometry, Immunohistochemistry (Frozen), SDS-Page, Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cancer, Embryonic Stem Cell Markers, Ovarian Carcinoma Cell Markers, Stem Cell Markers
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: Alkaline phosphatase Regan isozyme, alkaline phosphatase, placental, alkaline phosphatase, placental (Regan isozyme), alkaline phosphatase, placental type, alkaline phosphomonoesterase, ALP, EC 3.1.3.1, FLJ61142, glycerophosphatase, PALP, Placental alkaline phosphatase 1, PLAP, PLAP-1, Regan isozyme
• Gene Symbols: ALPP
• Isotype: IgG1 κ
• Purification Method: Protein A or G purified
• Test Specificity: PLAP is a tissue specific, trophoblast-derived, 70kDa, glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+ metallo-glycoprotein that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific AP's in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98% homologous. Non-tissue specific AP is found in kidney, liver and bone. This MAb binds equally well to all common allelic variants (S, F, FS and I) of PLAP and to some variants of AP from normal human testis. This MAb can be used as tracer antibody in ELISA to detect PLAP in serum of S, F, FS and I phenotypes.

• Clone: ALPP/516
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Frozen 1.0 - 2.0 ug/ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Accession No.: P05187
• Gene ID (Entrez): 250
• Immunogen: Recombinant full-length human ALPP protein
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 70 kDa

For Research Use Only