Molecular Probes™ Alkaline Phosphatase Live Stain 

Alkaline Phosphatase Live Stain

Manufacturer:  Molecular Probes™ A14353

Catalog No. A14353

Description

Alkaline Phosphatase Live Stain is a stem cell imaging product that allows users to differentially stain pluripotent stem cells (PSCs). The dye is a cell-permeable fluorescent substrate for alkaline phosphatase (AP) that is non-toxic to cells, diffusing out over the course of two hours. The Alkaline Phosphatase Live Stain consists of 50µL of a 500X fluorescein-based dye in DMSO, sufficient for staining two 24-well plates, four 6 cm dishes or two 10 cm dishes.

This Alkaline Phosphatase Live Stain allows you to:

Identify pluripotent stem cells with a strong signal above background

Stain PSCs typically in 20 minutes or less

Use the first alkaline phosphatase stain marketed that maintains cell viability

Alkaline Phosphatase Pluripotent Stem Cell Detection Method
AP is a phenotypic marker of PSCs, including undifferentiated embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), and embryonic germ cells (EGCs). While AP is expressed in most cell types, its expression is highly elevated in PSCs. AP staining has therefore been used to differentially stain PSCs to easily distinguish them from mouse embryonic fibroblasts (MEFs) used as feeders and parental fibroblasts commonly used in reprogramming experiments.

Screen for Pluripotency Without Sacrificing Precious Colonies
The Alkaline Phosphatase Live Stain is ideal for screening colonies during early stages of the reprogramming workflow since the stain maintains stem cell viability. It can also be used in later stages as a negative selection tool for identifying undifferentiated cells.

Stain Cells Quickly
The Alkaline Phosphatase Live Stain utilizes an easy, non-permanent, cell-viable protocol for identifying PSCs in your experiments. Simply dilute the dye in media, apply to cells, gently wash, and the cells are ready for fluorescent imaging.

For Research Use Only. Not for use in diagnostics procedures.

Specifications

• Quantity: 50μL
• Storage Requirements: –20°C
• For Use With (Equipment): For Floid™ Cell Imaging System, Fluorescence Microscope
• For Use With (Application): For cell viability, proliferation and function, stem cell analysis, stem cell differentiation, stem cell engineering and reprogramming
• Format: Liquid

• Description: Alkaline Phosphatase Live Stain, 50μL, Green, Liquid, 188 to 190°C Boiling Point, 18 to 19°C Melting Point, 87 to 89°C Flash Point, Fluorescent Detection Method, –20°C Storage, >85% HPLC Purity, For Cell Viability, Proliferation & Function
• Detection Method: Fluorescent
• Kit Contents: Contains 1 amber vial
• Product Type: Alkaline Phosphatase Live Stain
• Color: Green

For Research Use Only. Not for use in diagnostic procedures.