Invitrogen™ Alpha A Crystallin Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA1009

Catalog No. PA1009

Description

PA1-009 detects alpha-A crystallin from bovine and rat samples. This antibody does not detect the unphosphorylated form of the protein. PA1-009 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~20 kDa protein representing alpha-A crystallin from bovine lens samples. Immunohistochemical staining of alpha-A crystallin in rat thymus samples results in staining of epithelial reticular cells. The PA1-009 immunogen is purified alpha-A Crystallin from bovine lens. Reconstitute in 100 μL PBS to create a stock of 1 mg/mL.

Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products-alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted.

Specifications

• Antigen: Alpha A Crystallin
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CRYAA
• Gene Alias: [a]A-crystallin; Acry 1; Acry-1; alpha A crystallin; alpha crystallin A chain; alpha-A-crystallin; alpha-crystallin A chain; Alpha-crystallin A(1-151); Alpha-crystallin A(1-156); Alpha-crystallin A(1-157); Alpha-crystallin A(1-162); Alpha-crystallin A(1-163); Alpha-crystallin A(1-165); Alpha-crystallin A(1-168); Alpha-crystallin A(1-172); Alpha-crystallin A2 chain; Alpha-crystallin A2(1-162); Alpha-crystallin A2(1-168); Alpha-crystallin A2(1-172); CRYA 1; Crya1; Crya-1; CRYAA; CRYAA2; Crystallin Alpha 1; crystallin alpha A; crystallin, alpha 1; crystallin, alpha A; Crystallin, alpha polypeptide A; crystallin, alpha-1; CTRCT9; DAcry-1; Heat shock protein beta-4; HSPB 4; HSPB4; human alphaA-crystallin (CRYA1); lens opacity 18; LOC102724652; lop18; small heat shock protein; wu:fc06h04; zgc:92036
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 24273, 281718
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 30mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: P02470, P24623
• Gene Symbols: CRYAA
• Immunogen: Purified alpha-A Crystallin from bovine lens.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Rat, Bovine
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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