MilliporeSigma™ alpha-Actin, Cardiac Muscle, Mouse, Unlabeled, Clone: 22D3,

Catalog No.	Quantity	Conjugate
MABT823MI	100 μL	Unconjugated

Catalog No. MABT823MI Supplier MilliporeSigma™ Supplier No. MABT823

Description

Mouse Monoclonal Antibody

Actin, alpha cardiac muscle 1 (UniProt P68032; also known as Alpha-cardiac actin) is encoded by the ACTC1 (also known as ACTC, ASD5, CMD1R, CMH11, LVNC4) gene (Gene ID 70) in human. Actins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin is encoded by the ACTA1 gene, smooth muscle alpha-actin by the ACTA2 gene, cytoplasmic beta-actin by the ACTB gene, cardiac muscle alpha-actin by the ACTC1 gene, cytoplasmic gamma-actin by the ACTG1 gene, and smooth muscle gamma-actin by the ACTG2 (a.k.a. ACTA3) gene. Although actins show >90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expresson patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic - and -actins, are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt.

Specifications

• Antigen: alpha-Actin, Cardiac Muscle
• Applications: Immunocytochemistry, Immunofluorescence, Western Blot
• Classification: Monoclonal
• Clone: 22D3
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Purified mouse IgG1 in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
• Gene: ACTC1, ACTC, ASD5, CMD1R, CMH11, LVNC4
• Gene Accession No.: P68032
• Host Species: Mouse
• Immunogen: KLH-conjugated linear peptide corresponding to human mature alpha-cardiac actin N-terminal end sequence with an acetylated N-terminus.
• Purification Method: Protein G Purified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Cell Structure
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_005150.1
• Test Specificity: Clone 22D3 specifically detected alpha-cardiac actin in rat myocardium homogenates with no cross-reactivity toward - or -smooth muscle actin in rat aorta and chicken gizzard homogenates, cytoplasmic - or -actin in human platelet lysate, nor -skeletal actin in rat skeletal muscle homogenate (Driesen, R.B., et al. (2009). J. Cell. Mol. Med. 13(5):896-908).
• Target Species: Goat
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Form: Purified
• Isotype: IgG1 κ

For Research Use Only. Not for use in diagnostic procedures.