Invitrogen™ alpha Actinin 4 Monoclonal Antibody (7H6)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA1800

Catalog No. MA1800

Description

MA1-800 detects the alpha Actinin 4 in human and mouse cells. MA1-800 has been successfully used in Western blot, and immunofluorescence procedures. MA1-800 detects alpha Actinin 4 which has a predicted molecular weight of approximately 105 kDa. Under reducing conditions, MA1-800 detects a monomer of alpha Actinin 4 at approximately 52.5 kDa. The MA1-800 immunogen is a synthetic peptide corresponding to residues A(884) P Y Q G P D A V P G A L D(897) of human alpha Actin 4.

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.

Specifications

• Antigen: alpha Actinin 4
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: O43707, P57780
• Gene Symbols: Actn4
• Immunogen: Synthetic peptide corresponding to residues A(884) P Y Q G P D A V P G A L D(897) of human alpha Actinin 4.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
• Clone: 7H6
• Conjugate: Unconjugated
• Gene: Actn4
• Gene Alias: actinin alpha 4; ACTININ-4; actinin-Alpha-4; Actn4; alpha actinin 4; alpha-actinin-4; C77391; F-actin cross-linking protein; focal segmental glomerulosclerosis 1; FSGS; FSGS1; non-muscle alpha-actinin 4
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 60595, 81
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid