Invitrogen™ alpha Actinin 4 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA585368

Catalog No. PIPA585368

Description

Keep as concentrated solution. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (98%), Xenopus laevis (97%), Chicken (98%), Rhesus Monkey (100%), Bovine (100%). Positive Control: 293T, A431, HeLa, HepG2, NIH-3T3, JC, PC-12. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.

Specifications

• Antigen: alpha Actinin 4
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Actn4
• Gene Alias: actinin alpha 4; ACTININ-4; actinin-Alpha-4; Actn4; alpha actinin 4; alpha-actinin-4; C77391; F-actin cross-linking protein; focal segmental glomerulosclerosis 1; FSGS; FSGS1; non-muscle alpha-actinin 4
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 60595, 63836, 81
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry, Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: O43707, P57780, Q9QXQ0
• Gene Symbols: Actn4
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human alpha Actinin 4.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG