Invitrogen™ alpha-ENaC Monoclonal Antibody (2G4), FITC

Catalog No.	Quantity
PIMA545350	100 μg

Catalog No. PIMA545350

Supplier: Invitrogen™ MA545350

Description

Mouse Monoclonal Antibody

A 1:1,000 dilution of MA5-45350 was sufficient for detection of ENaC alpha in 15 μg of Mouse whole kidney lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 85kDa.

Epithelial sodium channels are amiloride-sensitive members of the degenerin/epithelial sodium channel (Deg/ENaC) superfamily of ion channels. Members of this superfamily of ion channels share organizational similarity in that they all possess two short intracellular amino and carboxyl termini, two short membrane spanning segments, and a large extracellular loop with a conserved cysteine-rich region. There are three homologous isoforms of the ENaC (alpha, beta, and gamma) protein. ENaC in the kidney, lung, and colon plays an essential role in trans-epithelial sodium and fluid balance. ENaC also mediates aldosterone-dependent sodium reabsorption in the distal nephron of the kidney, thus regulating blood pressure. ENaC is thought to be regulated, in part, through association with the cystic fibrosis transmembrane conductance regulator (CFTR) chloride ion channel. Gain-of-function mutations in beta- or gamma-ENaC can cause severe arterial hypertension (Liddel's syndrome) and loss-of-function mutations in alpha- or beta-ENaC causes pseudohypoaldosteronism (PHA-1).

Specifications

• Antigen: alpha-ENaC
• Applications: Immunohistochemistry, Immunohistochemistry (Paraffin), Western Blot
• Classification: Monoclonal
• Clone: 2G4
• Concentration: 1 mg/mL
• Conjugate: FITC
• Formulation: PBS, 9.1mM sodium bicarbonate with 640.91mM DMSO, 136.36mM ethanolamine and no preservative; pH 7.4
• Gene: SCNN1A
• Gene Accession No.: P37089, Q61180
• Gene Alias: alpha ENaC-2; alpha-ENaC; Alpha-NaCH; amiloride-sensitive epithelial sodium channel; amiloride-sensitive epithelial sodium channel alpha subunit; amiloride-sensitive sodium channel subunit alpha; amiloride-sensitive sodium channel subunit alpha 2; BESC2; ENaC; ENaC alpha; ENaCa; ENaCalpha; Epithelial Na(+) channel subunit alpha; epithelial sodium channel alpha subunit; FLJ21883; mENaC; nasal epithelial sodium channel alpha subunit; nonvoltage-gated sodium channel 1 subunit alpha; Renac; SCNEA; SCNN1; Scnn1a; sodium channel epithelial 1 alpha subunit; sodium channel, non voltage gated 1 alpha subunit; sodium channel, nonvoltage-gated 1 alpha; sodium channel, non-voltage-gated 1 alpha subunit; sodium channel, nonvoltage-gated, type I, alpha; sodium channel, nonvoltage-gated, type I, alpha polypeptide
• Gene Symbols: SCNN1A
• Host Species: Mouse
• Immunogen: Synthetic peptide from the N-terminal of Rat ENaC alpha (aa. 46-68).
• Purification Method: Protein G
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 20276, 25122
• Target Species: Mouse, Rat
• Content And Storage: 4°C, store in dark
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b