alpha GalCer:CD1d Complex Monoclonal Antibody (L363), Functional Grade, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
16-201-982	Invitrogen™ 16201982	100 μg

Description

Description: This L363 monoclonal antibody reacts with alpha-galactosylceramide bound to the mouse MHC class I-like protein CD1d. This complex is recognized by invariant natural killer T (iNKT) cells, a subset of T lymphocytes that expresses the invariant TCR alpha chain Valpha14Jalpha18. Activation of iNKT cells leads to production of Th1 and Th2-associated cytokines as well as dendritic cell maturation. The L363 antibody has been reported to neutralize iNKT cell activation by alpha-galactosylceramide:CD1d complexes. Applications Reported: This L363 antibody has been reported for use in functional assays. Applications Tested: This L363 antibody has been tested by flow cytometric analysis of alpha-galactosylcermide-loaded mouse CD1d-transfected cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

This complex is recognized by invariant natural killer T (iNKT) cells, a subset of T lymphocytes that expresses the invariant TCR alpha chain Va14Ja18. Activation of iNKT cells leads to production of Th1 and Th2-associated cytokines as well as dendritic cell maturation.

Specifications

• Antigen: alpha GalCer:CD1d Complex
• Concentration: 1 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with no preservative; pH 7.2
• Gene Alias: 3'-phosphoadenosine-5'-phosphosulfate:Galcer sulfotransferase; 3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase; 3'-phosphoadenylylsulfate-galactosylceramide 3'-sulfotransferase; AI747460; Antigen-presenting glycoprotein CD1d1; CD1.1; Cd1a; Cd1d; CD1d.1; Cd1d1; CD1d1 antigen; cerebroside sulfotransferase; Cst; Gal3st1; galactose-3-O-sulfotransferase 1; galactosylceramide sulfotransferase; GalCer sulfotransferase; Gcst; Ly-38; T-cell surface glycoprotein CD1d1
• Gene Symbols: Cd1d1, Gal3st1
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Gene: Cd1d1

• Clone: L363
• Applications: Flow Cytometry, Functional Assay
• Conjugate: Functional Grade
• Host Species: Mouse
• Target Species: Mouse
• Gene Accession No.: P11609, Q9JHE4
• Gene ID (Entrez): 12479, 53897
• Isotype: IgG2a κ
• Purification Method: Affinity Chromatography
• Product Type: Antibody

For Research Use Only.