Description: This L363 monoclonal antibody reacts with alpha-galactosylceramide bound to the mouse MHC class I-like protein CD1d. This complex is recognized by invariant natural killer T (iNKT) cells, a subset of T lymphocytes that expresses the invariant TCR alpha chain Valpha14Jalpha18. Activation of iNKT cells leads to production of Th1 and Th2-associated cytokines as well as dendritic cell maturation. The L363 antibody has been reported to neutralize iNKT cell activation by alpha-galactosylceramide:CD1d complexes. Applications Reported: This L363 antibody has been reported for use in functional assays. Applications Tested: This L363 antibody has been tested by flow cytometric analysis of alpha-galactosylcermide-loaded mouse CD1d-transfected cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC. This complex is recognized by invariant natural killer T (iNKT) cells, a subset of T lymphocytes that expresses the invariant TCR alpha chain Va14Ja18. Activation of iNKT cells leads to production of Th1 and Th2-associated cytokines as well as dendritic cell maturation.
|alpha GalCer:CD1d Complex|
|Flow Cytometry, Functional Assay|
|PBS with no preservative; pH 7.2|
For Research Use Only.
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