alpha-Smooth Muscle Actin Antibody (SPM332), FITC, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP234760F

Catalog No. NB006588

Description

alpha-Smooth Muscle Actin Monoclonal specifically detects alpha-Smooth Muscle Actin in Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Feline, Guinea Pig, Goat, Baboon, Chimpanzee, Monkey, Rabbit, Sheep samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Flow (Intracellular).

Specifications

• Antigen: alpha-Smooth Muscle Actin
• Classification: Monoclonal
• Conjugate: FITC
• Formulation: PBS with 0.05% Sodium Azide
• Gene Symbols: ACTA2
• Immunogen: N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH. (Uniprot: P62736)
• Quantity: 0.1 mL
• Research Discipline: Angiogenesis, Cancer, Cardiovascular Biology, Cell Biology, Cellular Markers, Developmental Biology, Extracellular Matrix, Signal Transduction, Stem Cell Markers
• Test Specificity: Actin is a major component of the cytoskeleton and is present in most cell types. This monoclonal antibody is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This monoclonal antibody does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
• Content And Storage: Store at 4°C in the dark.

• Applications: Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: SPM332
• Dilution: Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Flow (Intracellular)
• Gene Alias: AAT6, actin, alpha 2, smooth muscle, aorta, actin, aortic smooth muscle, ACTSA, ACTVS, alpha 2 actin, alpha SMA, alpha smooth muscle actin, alpha-actin-2, alpha-SMA, a-sma, Cell growth-inhibiting gene 46 protein, growth-inhibiting gene 46, sma, smooth muscle actin
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Pig, Bovine, Canine, Chicken, Feline, Guinea Pig, Goat, Primate, Baboon, Rabbit, Sheep
• Isotype: IgG2a κ