Invitrogen™ Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4 (asm-1)), Biotin

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA511544

Catalog No. PIMA511544

Description

MA5-11544 targets Actin Smooth Muscle in WB, immunofluorescence and immunohistochemistry applications and shows reactivity with cow, chicken, human, mouse, non-human primate, rabbit, and rat samples. The MA5-11544 immunogen is n-terminal decapeptide of alpha-smooth muscle isoform of actin; acetylated at the N-terminus.

Smooth Muscle Actin belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actin being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. In particular, smooth muscle actin is an alpha actin that is found in skeletal muscle. Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. Smooth muscle actin is found on smooth muscle vessel walls, gut wall, myometrium, myoepithelial cells in breast and salivary glands. Defects in the smooth muscle actin gene cause aortic aneurysm familial thoracic type 6. Actin isoforms differ slightly in their N-terminus and the sequences of each are perfectly conserved in higher vertebrates. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition, it has also been shown that smooth muscle actin appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases. Multiple alternatively spliced variants of smooth muscle actin have been identified.

Specifications

• Antigen: Alpha-Smooth Muscle Actin
• Classification: Monoclonal
• Concentration: 40 μg/mL
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.4
• Gene Accession No.: P08023, P62736, P62737, P62738, P62739, P62740
• Gene Symbols: ACTA2
• Immunogen: N-terminal decapeptide of alpha-smooth muscle isoform of actin; acetylated at the N-terminus.
• Quantity: 500 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Rabbit, Monkey, Bovine, Chicken
• Product Type: Antibody
• Isotype: IgG2a κ

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: 1A4 (asm-1)
• Conjugate: Biotin
• Gene: ACTA2
• Gene Alias: 0610041G09Rik; AAT6; ACT-4; acta2; acta2.L; acta2.S; actin; Actin Alpha 2 Smooth Muscle Aorta; actin alpha 2, smooth muscle; Actin Vascular Smooth Muscle; actin, alpha 2, smooth muscle, aorta; actin, alpha 2, smooth muscle, aorta L homeolog; actin, alpha 2, smooth muscle, aorta S homeolog; actin, alpha, vascular smooth muscle; actin, aortic smooth muscle; Actin, aortic smooth muscle, intermediate form; ACTSA; ACTVS; alpha actin 2; Alpha Cardiac Actin; alpha smooth muscle actin; alpha-actin; Alpha-actin-2; alpha-cardiac actin; alphaSMA; alpha-SM-actin; alpha-smooth muscle actin; Aortic Smooth Muscle; asma; a-sma; cell growth-inhibiting gene 46 protein; GIG46; Growth Inhibiting Gene 46; hypothetical protein LOC515610; LOW QUALITY PROTEIN: actin, aortic smooth muscle; mymy5; SM actin; sma; SMactin; sm-actin; SMalphaA; smooth muscle; smooth muscle alpha-actin; vascular smooth muscle alpha-actin; wu:fb63d03; XELAEV_18034370mg
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 100009271, 11475, 423787, 515610, 59, 81633
• Content And Storage: 4°C
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov