Invitrogen™ alpha Synuclein Monoclonal Antibody (10H7), FITC

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA545841

Catalog No. PIMA545841

Description

A 1:1,000 dilution of MA5-45841 was sufficient for detection of Alpha Synuclein in 15 μg of human brain cell lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. Binds within the 101-130 aa region of human alpha synuclein, determined using Dot blot and ELISA with corresponding peptides. Detects approximately 14 kDa band corresponding to Alpha synuclein. Band at approximately 40 kDa is a trimer. Does not detect truncated alpha Synuclein (aa 1-114).

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. These proteins are abundant in the brain and play a role in regulating synaptic transmission and membrane trafficking. Alpha- and beta-synuclein have been shown to selectively inhibit phospholipase D2, suggesting that they may also be involved in signaling pathways. Defects in the SNCA gene, which encodes alpha-synuclein, have been implicated in the pathogenesis of Parkinson's disease. Additionally, alpha-synuclein peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Multiple alternatively spliced transcripts encoding different isoforms have been identified for this gene. Diseases associated with SNCA include Parkinson Disease 1, Autosomal Dominant and Dementia, Lewy Body.

Specifications

• Antigen: alpha Synuclein
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS, 9.1mM sodium bicarbonate with 640.91mM DMSO, 136.36mM ethanolamine and no preservative; pH 7.4
• Gene Accession No.: O55042, P37377, P37840
• Gene Symbols: SNCA
• Immunogen: Mouse alpha synuclein aggregate.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: Dot Blot, ELISA, Western Blot, Immunocytochemistry
• Clone: 10H7
• Conjugate: FITC
• Gene: SNCA
• Gene Alias: Alpha synuclein; alphaSYN; alpha-synuclein; NACP; non A-beta component of AD amyloid; non-A beta component of AD amyloid; non-A4 component of amyloid; Non-A4 component of amyloid precursor; PARK1; PARK4; PD1; Snca; Syn; synuclein alpha; synuclein alpha-140; synuclein, alpha; synuclein, alpha (non A4 component of amyloid precursor)
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 20617, 29219, 6622
• Content And Storage: 4°C, store in dark
• Form: Liquid