alpha-Tubulin Polyclonal Antibody, Rockland™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-199-1835	Rockland Immunochemicals 600401880	200 μg
50-199-1836	Rockland Immunochemicals 600401880S	25 μL

Description

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Anti-Tubulin Loading Control Antibody is directed against human alpha Tubulin protein. A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

Specifications

• Antigen: alpha-Tubulin
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Target Species: Bovine, Chicken, Human, Mouse, Rat
• Gene Accession No.: P05213, P0DPH7, P68363, P68366, P68368, P68369, P68370, P68373, P81947, P81948, Q32KN8, Q3ZCJ7, Q5XIF6, Q6AYZ1, Q6P9V9, Q6PEY2, Q71U36, Q9BQE3
• Gene ID (Entrez): 100857247, 100857858, 10376, 112714, 22142, 22143, 22145, 22146, 300218, 316531, 404053, 421169, 429035, 500929, 504244, 534900, 539882, 64158, 7277, 7278, 777775, 7846, 84790
• Primary or Secondary: Primary
• Product Type: Antibody

• Concentration: 1.1 mg/mL
• Classification: Polyclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Alias: 1t; a1t; ALPHA 84C; alpha tubulin; alpha tubulin 84B; alpha[[1]] tubulin; alpha[[1]]-tubulin; alpha1; alpha1 tubulin; alpha1t; alpha1tub; alpha1tub84B; alpha1-tubulin; alpha84B; alphaT; alphat-1; alphatub; alpha-Tub; alphaTub1; alphatub84; alphaTub84B; alpha-tub84B; alphaTub84B-PA; alphatubulin; alpha-tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3D; Alpha-tubulin 3E; Alpha-tubulin 4; Alpha-tubulin 6; alphaTubulin 84B; alpha-tubulin 84B; alpha-Tubulin at 84B; Alpha-tubulin I; Alpha-tubulin II; Alpha-tubulin III; Alpha-tubulin isotype M-alpha-1; Alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; Alpha-tubulin isotype M-alpha-6; alpha-tubulin TUB1; Alpha-tubulin ubiquitous; alphaTubulin84B; alpha-tubulin84B; ALS22; anon-EST:Liang-1.59; anon-EST:Liang-2.30; aTub84B; a-tub84B; bA408E5.3; b-alpha-1; bcm948; BEST:LD32507; BOS_16828; BOS_16831; BOS_2117; BOS_5053; BOS_5055; cb944; CG1913; CG1913 gene product from
• Gene Symbols: Tuba1a, Tuba1b, TUBA1C, TUBA3C, TUBA3E, TUBA4A
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Gene: Tuba1a