Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

Catalog Number	Mfr. No.	Volume	Quantity
A25599	Invitrogen™ A25599	22 mL	400 Preps
A25600	Invitrogen™ A25600	5.5 mL	100 Preps
A25601	Invitrogen™ A25601	2.2 mL	20 Preps

Description

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

This new addition to the family includes a Power SYBR™ Green 1-Step RT-PCR master mix. The 1-step format of the RT-PCR step streamlines the Cells-to- CT™ 'sample to answer' workflow more than ever without compromising sensitivity and specificity and at an extraordinary value.

The Cells-to-CT 1-Step Power SYBR™ Green Kit is:

• Complete—optimized workflow includes cell lysis reagents with gDNA removal and Power SYBR™ Green 1-Step RT-PCR Master Mix
• Faster—7-minute sample prep, including DNase treatment, versus 30-60 minutes for traditional RNA purification
• Simple—12 steps total (sample prep and real-time RT-PCR combined)
• Robust—perform gene expression analysis on 10–100,000 cells per sample; results equivalent to those from purified RNA
• Affordable—contains sufficient reagents to perform 5 real-time RT-PCRs for each cell sample prepared; study multiple targets per sample at an affordable price

Simple 7-minute Sample Preparation, Then Ready for Real-time RT-PCR

Whether you are using plates or tubes, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit uses a simple 7-minute sample preparation procedure. The lysis technology is designed for 10–100,000 cultured cells/sample. Cells are washed in PBS and lysed in solution for 5 minutes at room temperature with a simultaneous DNase treatment. Lysis is terminated at room temperature by a 2-minute incubation with Stop Solution. The lysate is now ready to analyze on your real-time PCR instrument. Simply add it to a real-time RT-PCR master mix containing the primer sets for your genes of interest. Alternatively, the lysate can be stored at –20°C. Because samples can be processed directly in culture wells (96 or 384 wells), sample handling and the potential for sample loss or transfer error are minimized, facilitating rapid, high-throughput processing. Unlike old-fashioned, multi-step RNA isolation protocols, no heating, washing, or centrifugation steps are required. The kit greatly simplifies a laborious 30–60 minute process and reduces it to 7 minutes.

Completely Validated, Completely Affordable

All components of the Cells-to-CT™ 1-Step Power SYBR™ Green Kit have been optimized for consistent and reliable performance. This removes the guesswork involved in assembling separate sample preparation and real-time 1-step RT-PCR master mixes.

For added quality assurance, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit has been validated with a set of primers for common gene targets and shows performance equivalent to that obtained with purified RNA.

When you add it all up, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit is the best value for your gene expression experiments. With enough reagents to perform 5 real-time RT-PCRs per sample, the savings are substantial compared to buying master mix and an RNA purification kit separately to do the same work.

Accessory Product

The SYBR™ Green Cells-to-CT™ Control Kit (Cat. No. 4402959) was designed for use with SYBR™ Green Cells-to-CT™ products. This kit contains an endogenous control primer set (ACTB) to normalize for sample loading variability and an artificial XenoRNA™ control and corresponding primer set to monitor the effects of PCR inhibition.

Specifications

• Detection Method: SYBR
• GC-Rich PCR Performance: High
• Reaction Speed: 7 min.
• Green Features: Fewer resources used and less waste, less hazardous
• Product Type: 1-Step qPCR kit
• Reagent Type: Cell Lysis
• Starting Material: 10-100,000 Cells

• Format: Tube
• PCR Method: 1-step RT-qPCR
• For Use With (Equipment): 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
• Product Line: Cells-to-CT™, Power SYBR™ Green
• For Use With (Application): Gene Expression
• Sample Type: Mammalian Cells

For Research Use Only. Not for use in diagnostic procedures.