DescriptionAmbion In Vivo siRNA Controls
What are Ambion In Vivo siRNAs?
Ambion In Vivo siRNAs are designed using the proven Silencer Select algorithm and incorporate chemical modifications for superior serum stability with in vivo delivery. The added serum stability does not compromise the performance of the siRNAs. Ambion In Vivo siRNAs have been shown to be non-toxic in vivo (mouse) and non-immunogenic (cell-based assays). In cell-based assays, Ambion In Vivo siRNAs exhibit equivalent or better potency than Silencer Select siRNAs. The result is better confidence in your in vivo RNAi experiments, better knockdown when paired with an efficient delivery solution, and the assurance of limited non-specific effects.
Negative Control siRNAs
Negative control siRNAssiRNAs with sequences that do not target any gene productare essential for determining the effects of siRNA delivery and for providing a baseline to compare siRNA-treated samples. Ambion In Vivo siRNAs and have no significant sequence similarity to mouse, rat, or human gene sequences. These negative control siRNAs have been tested by microarray analysis and shown to have minimal effects on gene expression. In addition, these negative control siRNA has been tested in multi-parametric cell-based assays and are proven to have no significant effect on cell proliferation, viability, or morphology in the cell lines tested.
Ambion synthesizes and purifies each Ambion In Vivo siRNA in state-of-the-art facilities to meet the highest quality standards. As part of our rigorous quality control procedures, each RNA oligonucleotide is analyzed by MALDI-TOF mass spectrometry, and analytical HPLC is used to monitor purity. To provide the utmost in quality, we also assess each annealed siRNA by gel electrophoresis to confirm that the strands anneal properly. The result is premium-quality siRNA that is purified and ready to use.
In Vivo Ready Controls
Our In Vivo Ready Control siRNAs are subjected to that extra level of purification and testing required for the introduction of siRNAs into animals. After HPLC purification and annealing, each siRNA is further purified utilizing a process that removes excess salt via a semi-permeable membrane. The result is highly pure siRNA with minimal salt content, suitable for in vivo applications. In vivo siRNAs are then sterile filtered, and tested for the presence of endotoxin. At concentrations of 50µM in presence of deionized water, in vivo Ready siRNAs contain <5.0 mM Na+, <0.06 mM K+, and <0.02 mM Mg2+.
|The siRNA (annealed) is shipped at ambient temperature as dried down oligonucleotides in a single tube, along with 1.75mL nuclease-free water for resuspension|
|Ambion™ In-vivo Negative Control #1 siRNA|
For Research Use Only. Not for use in diagnostic procedures.
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