Invitrogen™ MagMAX™-96 Total RNA Isolation Kit  

Magnetic bead-based technology to purify total RNA

Manufacturer:  Invitrogen™ AM1830

Catalog No. AM1830

Includes: Kit contents: 1 Processing Plate and Lid; 11mL Lysis/Binding Solution Concentrate; 18mL Wash Solution 1 Concentrate; 55mL Wash Solution 2 Concentrate; 12mL RNA Rebinding Concentrate; 10mL Elution Buffer; 6mL MagMAX TURBO DNase Buffer; 1.1mL RNA Binding Beads; 1.1mL Lysis/Binding Enhancer; 110µL TURBO DNase. Contains enough reagents to isolate RNA from 96 samples.

Description

The MagMAX-96 Total RNA Isolation Kit is designed for the rapid, moderate-throughput purification of RNA from animal and plant cells and tissue. RNA purified using the kit is ideal for PCR and RT-PCR applications. The kit uses magnetic bead–based purification and includes sufficient reagents for 96 reactions.
Benefits of MagMAX magnetic bead–based purification Magnetic beads offer many benefits compared to other technologies for isolating RNA. Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples. Since only a small volume of magnetic beads is needed for high-efficiency binding, the bound RNA can be eluted in just 20–50µL of nuclease-free water, concentrating RNA from large, dilute samples.
Accommodates diverse sample types The kit accommodates diverse biological samples and a broad range of cell and tissue input amounts. The kit is optimized for high-throughput isolation of RNA from 25 cells to 2 x 10⁶ cells, as well as small plant and mammalian tissue samples (up to 10mg). The protocol is fully amenable to automation. Detailed guidelines for general automation are included with the kit.

• Enhanced lysis/binding conditions to accommodate larger sample inputs
• Reagents for TURBO DNase™ treatment included to minimize DNA contamination and preserve RNA integrity
• Includes protocol for RNA isolation from plant tissues
• 96 reactions per kit suitable for moderate throughput
• Automated protocols, high-throughput compatible users

Accessory products Lysis/Binding Solution Concentrate, Wash Solution 1 Concentrate, and Wash Solution 2 Concentrate are components of the kit that may also be purchased separately. The Plant RNA Isolation Aid can be used in conjunction with the MagMAX-96 Total RNA Isolation Kit for the isolation of RNA from plant tissues.

Automated Nucleic Acid Purification, Automated Total RNA Isolation, DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues, Total RNA from Plant Cells

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Specifications

• Final Product Type: Total RNA
• For Use With (Equipment): MagMAX™ Express 24 and 96, Robotic Liquid Handling Systems, MagMAX™ Express-96 Standard Magnetic Particle Processor
• Sample Type: Mammalian Cells, Plant, Tissue (Mammalian)
• Isolation Technology: Magnetic Bead
• Content And Storage: Kit contents (storage):
  • 1 Processing Plate & Lid (room temperature)
  • 11 ml Lysis/Binding Solution Concentrate (room temperature)
  • 18 ml Wash Solution 1 Concentrate (room temperature)
  • 55 ml Wash Solution 2 Concentrate (room temperature)
  • 12 ml RNA Rebinding Concentrate (room temperature)
  • 10 ml Elution Buffer (4°C or room temperature)
  • 6 ml MagMAX™ TURBO™ DNase Buffer (4°C or room temperature)
  • 1.1 ml RNA Binding Beads (4°C)
  • 1.1 ml Lysis/Binding Enhancer (-20°C)
  • 110 µl TURBO™ DNase (-20°C)
  
  Contains enough reagents to isolate RNA from 96 samples.
• Starting Material Amount: Up to 10 mg plant tissue, Up to 2 x10^6 cells, Up to 5 mg animal tissue

• For Use With (Application): Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting, cDNA Library Construction
• Purification Target: Total RNA
• Format: 96-well plate
• Yield: 40 µg
• High-throughput Compatibility: High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
• Product Line: Ambion™, MagMAX™

For Research Use Only. Not for use in diagnostic procedures.