Invitrogen MagMAX-96 Total RNA Isolation Kit
Magnetic bead-based technology to purify total RNA
Manufacturer: Invitrogen AM1830
The MagMAX-96 Total RNA Isolation Kit is designed for the rapid, moderate-throughput purification of RNA from animal and plant cells and tissue. RNA purified using the kit is ideal for PCR and RT-PCR applications. The kit uses magnetic beadbased purification and includes sufficient reagents for 96 reactions.
Benefits of MagMAX magnetic beadbased purification Magnetic beads offer many benefits compared to other technologies for isolating RNA. Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples. Since only a small volume of magnetic beads is needed for high-efficiency binding, the bound RNA can be eluted in just 2050µL of nuclease-free water, concentrating RNA from large, dilute samples.
Accommodates diverse sample types The kit accommodates diverse biological samples and a broad range of cell and tissue input amounts. The kit is optimized for high-throughput isolation of RNA from 25 cells to 2 x 106 cells, as well as small plant and mammalian tissue samples (up to 10mg). The protocol is fully amenable to automation. Detailed guidelines for general automation are included with the kit.
- Enhanced lysis/binding conditions to accommodate larger sample inputs
- Reagents for TURBO DNase treatment included to minimize DNA contamination and preserve RNA integrity
- Includes protocol for RNA isolation from plant tissues
- 96 reactions per kit suitable for moderate throughput
- Automated protocols, high-throughput compatible users
Accessory products Lysis/Binding Solution Concentrate, Wash Solution 1 Concentrate, and Wash Solution 2 Concentrate are components of the kit that may also be purchased separately. The Plant RNA Isolation Aid can be used in conjunction with the MagMAX-96 Total RNA Isolation Kit for the isolation of RNA from plant tissues.
Automated Nucleic Acid Purification, Automated Total RNA Isolation, DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues, Total RNA from Plant Cells
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|MagMAX™ Express 24 and 96, Robotic Liquid Handling Systems, MagMAX™ Express-96 Standard Magnetic Particle Processor|
|Mammalian Cells, Plant, Tissue (Mammalian)|
|Kit contents (storage):
• 1 Processing Plate & Lid (room temperature)
• 11 ml Lysis/Binding Solution Concentrate (room temperature)
• 18 ml Wash Solution 1 Concentrate (room temperature)
• 55 ml Wash Solution 2 Concentrate (room temperature)
• 12 ml RNA Rebinding Concentrate (room temperature)
• 10 ml Elution Buffer (4°C or room temperature)
• 6 ml MagMAX™ TURBO™ DNase Buffer (4°C or room temperature)
• 1.1 ml RNA Binding Beads (4°C)
• 1.1 ml Lysis/Binding Enhancer (-20°C)
• 110 µl TURBO™ DNase (-20°C)
Contains enough reagents to isolate RNA from 96 samples.
|Up to 10 mg plant tissue, Up to 2 x10^6 cells, Up to 5 mg animal tissue|
|Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting, cDNA Library Construction|
|High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols|
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For Research Use Only. Not for use in diagnostic procedures.