Invitrogen™ PureLink™ RNA Mini Kit

The PureLink RNA Mini Kit is a column-based kit used to isolate high-quality total RNA from a wide variety of sample types in 20 minutes using standard laboratory equipment.

Supplier:  Invitrogen™ 12183018A

Catalog No. 12-183-018A

Description

Rapid and convenient column purification
The PureLink™ RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and tissue types to yield up to 1000 μg of purified RNA from a single extraction. High-quality total RNA can be obtained from mini- to midi-prep amounts of starting material with no genomic DNA contamination.

Simplified, nontoxic RNA purification
The PureLink™ RNA Mini Kit combines nontoxic guanidine-isothiocyanate lysis with the speed, purity, and ease-of-use of silica-membrane purification. The safe and easy procedure can typically be completed in less than 20 minutes without the need for hazardous phenol/chloroform extraction, CsCl centrifugation, LiCl, or alcohol precipitation.

• Obtain high-yield, high-integrity RNA
• Rapid, non-hazardous RNA purification capabilities
• Ready-to-use RNA for many downstream applications

Specifications

• Content And Storage: • 125 mL Lysis Buffer; store at room temperature
  • 50 mL Wash Buffer I; room temperature
  • 15 mL Wash Buffer II; room temperature
  • 15.5 mL RNase-free Water; room temperature
  • 50 PureLink Spin Cartridges with Collection Tubes; room temperature
  • 50 Collection Tubes; room temperature
  • 50 Recovery Tubes; room temperature
• Purification Time: 20 min. (After sample homogenization)
• Sample Type: Bacteria, Blood, Cells, Plant, Tissue, Yeast, Enzymatic Reactions
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Quantity: 50 Preps
• Shipping Condition: Room Temperature
• Yield: Up to 350 μg

• Isolation Technology: Silica Spin Column
• Elution Volume: 30–300 μL
• Final Product Type: Total RNA
• For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
• Scale: Mini
• Starting Material Amount: Bacteria: ≤10⁹ cells
  Blood: ≤0.2 mL
  Cells: ≤5 x 10⁷
  Plant: ≤250 mg
  Tissue: ≤200 mg
  Yeast: ≤5 x 10⁸ cells
  Enzymatic reactions: ≤1.2 mL

For Research Use Only. Not for use in diagnostic procedures.