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Invitrogen™ PureLink™ RNA Mini Kit Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol

Manufacturer:  Invitrogen™ 12183025

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Catalog No. 12-183-025


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Description

Description

Rapid and convenient column purification
The PureLink™ RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and tissue types to yield up to 1000µg of purified RNA from a single extraction. High-quality total RNA can be obtained from mini- to midi-prep amounts of starting material with no genomic DNA contamination.

Simplified, nontoxic RNA purification
The PureLink™ RNA Mini Kit combines nontoxic guanidine-isothiocyanate lysis with the speed, purity, and ease-of-use of silica-membrane purification. The safe and easy procedure can typically be completed in less than 20 minutes without the need for hazardous phenol/chloroform extraction, CsCl centrifugation, LiCl, or alcohol precipitation.

  • Obtain high-yield, high-integrity RNA
  • Rapid, non-hazardous RNA purification capabilities
  • Ready-to-use RNA for many downstream applications

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Specifications

Specifications

Any Tissue Type, Bacterial Culture, Cell Cultures, Plant, Tissue Homogenates, Cultured Cells, Yeast, Tissue Sections, Blood, Liquid Samples (e.g. Serum)
Microarray Analysis, Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting
Spin Column
250 preps
• Lysis Buffer (500 ml)
• Wash Buffer I (250 ml)
• Wash Buffer II (75 ml)
• RNase-Free Water (75 ml)
• Spin Cartridges with collection tubes (5 × 50 each)
• Collection Tubes (5 × 50 each)
• Recovery Tubes (5 × 50 each)

Store at room temperature.
30 µl
20 min
Room Temperature
PureLink™
Total RNA
Total RNA
Silica Spin Column
350 µg
100 µl
Not High-throughput Compatible (Manual)
Mini
5x10^6 to 1x10^8 cells, Up to 200 mg of tissue
PureLink™
Documents
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For Research Use Only. Not for use in diagnostic procedures.