Invitrogen™ RiboPure™ RNA Purification Kit, yeast

For the isolation of extremely high-quality RNA from yeast without the use of enzymatic pretreatments that may alter gene expression profiles.

Supplier:  Invitrogen™ AM1926

Catalog No. AM1926

Description

For the isolation of extremely high-quality RNA from yeast without the use of enzymatic pretreatments that may alter gene expression profiles. Each Invitrogen kit includes sufficient reagents for 50 purifications each from 10⁷ to 10⁸ yeast cells.
It has traditionally been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Many protocols use enzymatic pretreatments that may affect gene expression patterns. The RiboPure-Yeast RNA Isolation Kit uses no enzymatic pretreatments and thus does not alter expression profiles. It combines a simple and efficient glass bead/one step phenol–mediated disruption using Invitrogen's Vortex Bead Beater Adapter followed by a glass fiber filter– based RNA purification step. After DNase treatment with the included DNA-free™ Reagents, the resulting RNA is exceptionally pure containing little to no genomic DNA (less than 0.05%). The kit has been used extensively at Invitrogen to isolate high-quality RNA from Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris, and Candida albicans. The kit provides the highest yield of any method tested from each of these species.

• Highest yields of total RNA available
• No enzymatic pretreatment required
• Includes DNA-free™ Reagents

Not high-throughput compatible (Manual)

Applications:
DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Yeast

Specifications

• Content And Storage: • 50 1.5-mL Screw Cap Tubes; store at room temperature
  • 50 Filter Cartridges; room temperature
  • 100 Collection Tubes (2 mL); room temperature
  • 24 mL Lysis Buffer; 4°C
  • 2.4 mL 10% SDS; 4°C
  • 24 mL Phenol:Chloroform:IAA; 4°C
  • 95 mL Binding Buffer; 4°C
  • 35 mL Wash Solution 1; 4°C
  • 50 mL Wash Solution 2/3 (concentrated); 4°C
  • 5 mL Elution Solution; 4°C
  • 80 g Zirconia Beads; -20°C
  • 550 μL 10X DNase 1 Buffer; -20°C
  • 200 μL DNase 1; -20°C
  • 550 μL DNase Inactivation Reagent; -20°C
• Purification Time: 1.5 hr. (Including DNase treatment)
• Sample Type: Yeast
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Quantity: 50 Preps
• Starting Material Amount: Up to 10⁸ cells

• Isolation Technology: Spin Column (Glass Fiber Filter)
• Elution Volume: 50–100 μL
• Final Product Type: Total RNA
• For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
• Shipping Condition: Box 1: Dry Ice
  Box 2: Room Temperature
• Yield: Up to 300 μg

For Research Use Only. Not for use in diagnostic procedures.