Invitrogen™ TRIzol™ Reagent
Isolate RNA, DNA, and protein from the same sample
Manufacturer: Invitrogen™ 15596018
- Safety and Handling
- Product Certifications
TRIzol Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour.
Reliably purify RNA from multiple sample volumes and sources TRIzol Reagent performs well with small quantities of tissue (50–100mg) and cells (5 × 106), as well as with large quantities of tissue (≥1g) and cells (>107), and comes with prototcols for purification from samples of human, animal, plant, or bacterial origin. TRIzol Reagent maintains the integrity of the RNA due to highly effective inhibition of RNase activity while disrupting cells and dissolving cell components during sample homogenization. The simplicity of the TRIzol Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in 1 hour. Total RNA isolated by TRIzol Reagent is free of protein and DNA contamination.
Formulated for isolation of multiple molecular targets TRIzol Reagent allows you to perform sequential precipitation of RNA, DNA, and proteins from a single sample. After homogenizing the sample with TRIzol Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), and interphase and red lower organic layers (containing the DNA and proteins). RNA is precipitated from the aqueous layer with isopropanol. DNA is precipitated from the interphase/organic layer with ethanol. Protein is precipitated from the phenol-ethanol supernatant by isopropanol precipitation. The precipitated RNA, DNA, or protein is washed to remove impurities, and then resuspended for use in downstream applications.
- Permits the isolation of RNA, DNA, and protein from the same sample
- Offers superior lysis capability, even with difficult sample types
- Optimized formulations and protocols for tissues, cells, serum, virus, and bacteria
Not high-throughput compatible (Manual)
DNA and RNA Purification and Analysis, RNA Extraction, RNAi, Epigenetics and Non-Coding RNA Research, Total RNA Isolation, Total RNA from Animal Cells and Tissues, Total RNA from Bacterial Samples, Total RNA from Plant Cells, Total RNA from Yeast, miRNA and Non-Coding RNA Analysis, miRNA Isolation
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|Total RNA, Transcriptome RNA, micro RNA|
|Bacteria, Blood, Cells, Plant Samples, Tissue, Viral Samples, Yeast|
|Not High-throughput Compatible (Manual)|
|200mL TRIzol Reagent|
|Up to 1 g tissue, Up to 1 x 10^7 cells|
|Total RNA, Transcriptome RNA, miRNA|
|Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Nuclease Protection Assays, Northern Blotting, Cloning|
Safety and Handling
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For Research Use Only. Not for use in diagnostic procedures.